Table 2 RT-qPCR primers used to test the expression of kidney renin as a dehydration marker and vocal fold genes selected based on differentially regulated proteins

REN

Renin

XM_017347610.1

Fw

GACATAGTGACTGTGGGCGG

87

Rv

CTTGGCCAGCATGAAGGGTA

GAPDH*

Glyceraldehyde-3-phosphate dehydrogenase

NM_001082253.1

Fw

GTCGGAGTGAACGGATTTGG

81

Rv

GGCGACAACATCCACTTTGC

LAMC1

Laminin subunit gamma 1

XM_017339901.1

Fw

CCTTCGGAGACGAGGTGTTC

95

Rv

GTTGCACTTGCACCTGCCG

FBLN5

Fibulin 5

XM_002719596.3

Fw

GCTCTCTGCTCCCAACTACC

101

Rv

CACACTCGTCCACATCCACA

COL18A1

Collagen XVIII alpha-1chain/ Endostatin domain-containing protein

XM_008253234.2

Fw

GGTCAACCTCAAGGACGAGG

198

Rv

GTCTCGCAGTAGCTGTTGGT

MFAP4

Microfibril associated protein 4

XM_002722656.3

Fw

GGCAAGTGGACGGTTTTCC

71

Rv

TTGTAGTCATTCCAGCCCCG

FBN1

Fibrillin 1

XM_008269057.2

Fw

TCAATGGCTACCCCAAACGG

78

Rv

CAGACTGATCCTCAACAGTGG

MFAP5

Microfibril associated protein 5

XM_002712855.3

Fw

CAGGGAGTGGCTTTGTTCCT

182

Rv

CCAAGCGCATCCATTCAGC

MYL2

Myosin regulatory light chain 2

XM_002719750.3

Fw

GGGAGAAACTCAAGGGAGCG

95

Rv

GTAGTCGGCCTTCAGCACTC

DMD

Dystrophin

XM_017349731.1

Fw

TGCGGTTCAGAAGCAAAACG

170

Rv

TCTCATCAGGAGGCAGCTCT

SLMAP

Sarcolemmal membrane-associated protein

NM_001082348.2

Fw

GCACAGTCAGAAACTGAGGC

196

Rv

TCTGCAGTTGGGCTTGAAGA

LMNA

Lamin A/C

XM_008264325.1

Fw

CAAGACCCTCGACTCTGTGG

102

Rv

TTCTTGGTGTTGCGCGCTTT

NIF3L1

NIF3-like protein 1

XM_002712443.3

Fw

TCGGAGCCTGTACATCCAGA

180

Rv

CCTCACCATCAGTCCTAGCAG

HPRT1*

Hypoxanthine phosphoribosyltransferase 1

NM_001105671.1

Fw

GATGGTCAAGGTCGCAAGCC

73

Rv

TCCAACAAAGTCTGGCCTGT