Fig. 1

Heatmaps showing Spearman (left) and Pearson (middle) correlations between 19 epigenetic features and SpCas9 off-target cleavage activities. Red and blue colors represent positive and negative correlations, respectively. The 19 epigenetic features consists of six experimental epigenetic features (bolded) and 13 nucleosome organization-related scores. The first four rows in the heatmaps display cell line-specific correlations. The fifth and sixth row display correlations for off-target sites in gene body and non-gene body regions. The final row displays the overall correlation for the epigenetic features. (Right) Pie plot showing the dataset’s cell line composition including all cell lines that contribute more than \(1\%\) to the crisprSQL dataset