Fig. 4
From: Benchmarking full-length transcript single cell mRNA sequencing protocols
Comparative quality metrics from scRNA-seq data generated with NEB®, Takara®, G&T and SS3 protocols. A cDNA ng/yield following mRNA amplification in each protocol. B Library sizes millions. C Total nr. genes detected per single cell. D Percentage of reads mapping to endogenous genes per single cell. E Percentage of reads mapping to exogenous genes; ERCC spike ins per single cell. F Nr. Captured ERCC genes per single cell. G Percentage of reads mapped to mitochondrial (mt) genes per single cell. H Nr. Captured MT genes per singe cell. Each dot represents a single cell. Significance level cut-off: ns:p < 0.05, *:p < = 0.05, **:p < = 0.01, ***:p < = 0.001, ****:p < = 0.0001