Fig. 7
From: Benchmarking full-length transcript single cell mRNA sequencing protocols
Hands-on time per protocol. Gantt chart featuring estimated hands-on time of each step of each tested protocol. NEB® and SS3 protocol has approximately 6 h of hands-on time. Takara® protocol has approximately 5 h and 45 min of hands-on time, by combining RT and PCR amp. Experimentalist save time both on processing but also leaves option for leaving the plate in thermocycler overnight, potentially saving even more time. G&T protocol has the longest processing time at 8 h 30 min due predominantly to reagent preparation and the separation step between RNA and DNA. All times are estimates assuming a skilled experimentalist with no prior experience with the protocol