Benchmarking full-length transcript single cell mRNA sequencing protocols

BMC Genomics

Table 1 Table summarising pro et contra of droplet versus plate-based techniques

	Droplet-based	Plate-based
Detected genes/per cell [6]	200-5000	4000 ~ 8000
Detected cells [9]	1 k-10 k per run	1 cell per well
Processing time [9]	~ 9-10 h	~ 25 h
Type of RNA analyzed	3’ – or 5’end cDNA	Full length cDNA
Others	Require Immediate processing UMI-based	Enable collection months before processing

ISSN: 1471-2164