Fig. 1

Global transcriptome assessment of porcine MII oocytes and IVV embryos. A Schematic showing the preparation of porcine MII oocytes and IVV embryos for RNA-seq. For MII oocytes were collected by in vitro maturation; 2-cell, 4-cell, 8-cell and blastocyst embryos were collected by in vivo fertilization (IVV). RNA sequencing was performed using pools of 10 oocytes/embryos (3 replicates per group). B Principal component analysis was performed for all developmental stages with their replicates (MII, n = 3; 2-cell to blastocyst, n = 3). Each point represents a replicate. Developmental stages were clearly separated from each other and replicates within stages were grouped together. C Heatmaps showing the gene expression levels among oocytes, 2-cell, 4-cell, 8-cell and blastocyst embryos. Each column represents a replicate. D The scatter plots show the upregulation and downregulation DEGs between porcine IVV and SCNT embryo at the 2-cell, 4-cell and 8-cell stage embryos (FC > 2, FDR < 0.05). E Cluster analysis showing the gene expression levels form porcine MII oocytes to blastocysts