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Fig. 6 | BMC Genomics

Fig. 6

From: Profiling the transcriptomic signatures and identifying the patterns of zygotic genome activation – a comparative analysis between early porcine embryos and their counterparts in other three mammalian species

Fig. 6

Assessment of the development competence of porcine embryo after treatment with 10,058-F4. A Representative images of IVF embryos after culturing for 7 days by treatment with 10,058-F4 at 0, 0.5 uM,1 uM, 2.5 uM, 5 uM, 7.5 uM, 10 uM, 25 uM, 50 uM or 100 uM for 48 h, Scale bars, 50 mm. B Relative abundance of c-MYC (B) and ZGA-related genes ZSCAN4 (C), EIF1AX (D), USP26 (E) and YTHDC2 (F) in the porcine IVF embryos, quantities were normalized to GAPDH abundance. Data are presented as the mean ± standard deviation. *, P < 0.05; **, P < 0.01 between groups, as indicated. BLA, blastocyst. G Representative examples of fragmented nuclei (green), which are indicative of apoptosis, versus total nuclei (blue), Scale bar, 50 um

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