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Fig. 2 | BMC Genomics

Fig. 2

From: Comprehensive microRNA analysis across genome-edited colorectal cancer organoid models reveals miR-24 as a candidate regulator of cell survival

Fig. 2

Smad4 signaling is a major driver of miR-375-3p expression in mouse enteroids. A, B Heatmaps show the magnitude of change in miRNA expression relative to WT by subtracting rlog normalized miRNA expression for each enteroid sample by the rlog average WT expression. Heatmaps shown are for Group B and Group A as defined by DEGReport. Color intensity shows rlog normalized miRNA expression in each genetically modified enteroid sample subtracted from average WT. Color scale minimum saturates at -3 and maximum saturates at 3. C Normalized miR-375-3p expression from small RNA-seq in each genotype. D MiR-375-3p expression from RT-qPCR following 0, 0.5, or 1 ng/mL treatment of mouse enteroids with recombinant human TGF-B1. Significance in (C) and (D) determined according to two-tailed Welch t-test. *p < 0.05, **p < 0.01, ***p < 0.001

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