Fig. 3

Identification of differentially expressed miRNA regulators of gene expression across genetically modified mouse enteroid models. A Schematic of the strategy utilized to identify miRNAs differentially expressed across a broad range of genotypes (Created with BioRender.com). B Results of the strategy highlights 19 guide miRNAs that are significantly differentially expressed (DESeq2 p-adj < 0.05, baseMean > 500, > 1.5 × fold change) in mutant genotypes relative to WT. C As shown by the rlog normalized counts, 10/19 miRNAs highlighed in (B) are differentially expressed in the same direction when comparing mutant genotypes (with n > 1) to WT. In the case of miRNAs for which both paralogs were identified as differentially expressed, only one paralog is shown. D Heatmap showing log2 fold change for miRNAs shown in (C). Color intensity represents the log2 fold change relative to WT. E Heatmap showing -log10(p-value) of target site enrichment, calculated by miRhub (cons1) for each differentially expressed miRNA from (D), in the list of genes that are differentially expressed (DESeq2 p-adj < 0.05, baseMean > 500, > 1.5 × fold change) in the opposite direction of the miRNA