Fig. 2

UpSet plots, summarising unique and shared differentially expressed genes due to exposure to different treatments. Black dots in panel matrices correspond to different Venn diagram sections: connected dots indicate intersections of sets of differentially expressed genes; singular dots represent unique sets of differentially expressed genes. Vertical barplots summarise the number of upregulated (a) and downregulated (b) genes. Horizontal bars indicate the total number of regulated genes in each treatment combination. The set union of all upregulated and downregulated genes detected in salinity treatments represents 443 salinity genes, of which one gene was upregulated when increased salinity was combined with reduced flow but downregulated when all three stressors were applied (= 442 exclusively regulated genes)