Transcriptomic analysis reveals the dynamic changes of transcription factors during early development of chicken embryo

Table 1 List of primer sequences used in qRT-PCR

Gene	Primer	Accession No.	Product length (bp)	Annealing temperature (℃)
POU5F3	F: GGAGCAGTTTGCCAAGGA R: GAAGCGGCAGATGGTGGT	NM_001309372.2	124	61
NANOG	F: CAGGTGAAGACGTGGTTT R: AGCCCTGGTGAAATGTAG	NM_001146142.2	148	61
SOX10	F: TCTGAAGACCACCACTGCCTCTC R: CTTGACCTTGCCCATCTCTCCATTC	NM_204792.2	99	60
CDX2	F: AAACCAGGACGAAGGACAAATACCG R: GGTGATATAGCGGCTGTAGTGGAAC	NM_204311.2	92	60
ISL1	F: TCCGAGGGTCATCAGGGTTTGG R: TGGGTTGCTGCTGCTGAAGTTG	NM_205414.2	92	60
PAX6	F: CTATCCCGATGTGTTTGCGAGAGAG R: CTGGGAGTGTTGCTGGCTTGTC	NM_205066.2	150	61
SOX2	F: AAACCAAGACCCTGATGAAGA R: ATCCCATAGCCTCCGTTG	NM_205188.3	175	61
OLIG2	F: GCTTCAAGTCCTCGTCGTCGTC R: CGGCTCCGTCATCTGCTTCTTG	NM_001031526.1	80	61
SMAD3	F: AGACGGCACATCGGAAGAGGAG R: AATGGCACTGTCACTAAGGCACTC	NM_204475.2	81	61
GATA6	F: AGAGAGCACCAGTCCCGAAAGC R: ACACCAGTGATCCTGCCTGACG	NM_205420.2	121	61
SOX9	F: GCTGTGGAGGCTGCTGAATGAG R: CCTGCGTGGTTGGTACTTGTAGTC	NM_204281.2	112	60
HNF4A	F: GCCAACCTCAACACCTCCAACAG R: TCCTCACGCTCCTCCTGAAGAAG	NM_001030855.2	133	61
MKX	F: GAACACAGTCAGGCAACCAGACC R: CCATCTTCAGAGCACGAGTCATCAC	XM_015282064.4	120	60
THRB	F: AATCAGTGCCAGGAATGTCGCTTC R: GCCTCTTGCTGTCATCCAACACC	XM_015282064.4	82	61
NFIA	F: GGAGCCGTTCTATACAAGCCAAGG R: GAAGGCGAGGGACTGCTGAAAC	NM_205273.2	141	60
ZBTB16	F: TCCACCGCAACAGTCAGCATTAC R: CGTAGAGCAGGTCATCCAAGTCTTC	NM_001321488.2	126	61
GAPDH	F: GCCATCACAGCCACACAGA R: TTTCCCCACAGCCTTAGCA	NM_204305.2	120	60 / 61

ISSN: 1471-2164