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Table 6 first round PCR reaction procedure

From: Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose

Temperature

Duration

Cycle

98 °C

5 min

 

98 °C

30s

8×

50 °C

30s

72 °C

30s

98 °C

30s

25×

66 °C

30s

72 °C

30s

72 °C

5 min

4 °C

Hold

Â