Fig. 4

RH-seq reveals cutl-24 as a candidate gene at which inter-strain variation contributes to tunicamycin resistance. In a given panel, in each row the left-hand cartoon represents the region of cutl-24 in the hybrid genome (red line, ED3077 chromosome; blue line, N2 chromosome), and the triangle denotes the position of insertion of a Mos1 transposon as detected by transposon sequencing. The right-hand cell reports the log₂ of the abundance of the respective mutant, detected by sequencing, after development in tunicamycin, relative to the analogous quantity from development in untreated control conditions. The p-value reports the result of a two-tailed Mann-Whitney statistical test for a difference in the abundance after tunicamycin selection, relative to the abundance in an untreated control, of hemizygotes harboring transposon insertions in the two parents’ orthologs. Supplementary Table 4 reports Mos1 insertion positions and raw quantitation data, with the Benjamini-Hochberg method used to correct for multiple testing. Top, hemizygotes harboring transposon insertions in the N2 allele; bottom, hemizygotes harboring transposon insertions in the ED3077 allele