Fig. 1

Experimental overview. A Schematic representation of Grh binding to nucleosome-bound DNA and making regions accessible for subsequent TF binding. B Third instar larval imaginal wing discs were dissected from the Zhr and Z30 strains (diverged ~ 10,000 years ago) and their F₁ hybrid with three biological replicates per genotype. C Bar plot showing the number of single nucleotide variants at each Grh motif position across the Zhr/Z30 genome (bottom) aligned with the canonical Grh motif (top). D For one representative sample (Z30 replicate 1) heatmaps showing the read count across a window of each region and gene used for the analyses. E For the same sample shown in (D), screenshots from genome browser showing Cut&Run, ATAC-seq, and RNA-seq read counts at ddc (top) and wg (bottom)