Fig. 2

Schematic description of the Successive Coverage Values (SCV) methodology. a) This strategy is based on the assumption that for a given gene, the highest the coverage value, the less doubtful is the transcript prediction. b) All RNA-seq alignments are processed by the StringTie tool providing transcriptome annotation files in output. c) All transcriptome annotation files are compared gene by gene. First, transcript predictions are validated if they fully cover their associated CDS. Then, the average coverage from each validated prediction are compared and those above the most restrictive value are finally selected