Table 4 Primers used for the qRT-PCR validation
Gene | FORWARD PRIMER | RIGHT PRIMER | SIZE (BP) |
|---|---|---|---|
smad2–3 | GCTGGATGAGCTTGAGAAGG | ACGTGCGGTAATCCTTTACG | 207 |
smad4 | CAGATGCAGCAGCAAGCA | AGCCCTTGACGAAGCTGAG | 290 |
ANX12 | TATTGAGAACTGCCTGACTGCC | TGTCCCAAGGCCCTTCATTG | 97 |
CHK1 | GCTGGTGAAGAGGATGACGC | CTCCTGTCCGTGGTGGAGAT | 144 |
MCM3 | CCGCGACCAAGAAGACCATC | CCTTGTAGACCGAGAGGCCA | 138 |
CCNA2 | GCTGAGCTTGCACCTCTTCA | CCTCCCCAGTGAGAAGGATGT | 134 |
MAD2 | TTCACCCGCGTCCAGAAGTA | GCACTTGCAGAGCCACTCTT | 111 |
CDK1 | ATGGCCTGATGTGGAGTCCC | AAAGCAGATCAAGCCCATCTTCAT | 113 |
CCND1 | CGAGCCTGCCAAGAACAGAT | GTGTGCAGGAAAGGTCTGCT | 118 |
FGF12 | AGCTCGGATGTTTTCACACC | GCGTCCTTGTTTTTCTCCAA | 258 |
MMP3 | GGACATGATGAAGCAGCCCA | TCGTCCACATCAGCTTGCAG | 144 |
PDGFA | GCCTTTGGCTTTATTGTGGA | GGTTTCCTTCACATCGGAGA | 199 |