Table 3 List of primers used for qRT-PCR.
Gene | Primer name | Sequence (5’-3’) | Amplicon size (bp) |
|---|---|---|---|
β-actin | β-actin-F β-actin-R | TACGAGCTGCCTGACGGACA GGCTGTGATCTCCTTCTGCA | 240 bp |
hipk1 | hipk1-F hipk1-R | GGCCAGATTGAGGTGGGTATC AATACCAGGCATGTGTGACCTT | 113Â bp |
epha2 | epha2-F epha2-R | GATGGACACGCGTGGATCAAA CTCCAAACGACCTCGCTTCA | 106Â bp |
slc7a5 | slc7a5-F slc7a5-R | AAGCCGATCGCACACTCCTT AGCGACCGATCAAGGTGAATA | 109Â bp |
acsl4 | acsl4-F acsl4-R | AAAAAGTGCACACAGAAGGCTAC CAATGGGCCGTTCCATATTCTC | 102Â bp |
nol6 | nol6-F nol6-R | GCCATTGACTAGGGTGAGATGT CGGCATGTACTATGATGCCCT | 118Â bp |
timp2 | timp2-F timp2-R | GTGCAGCGGATGATCTTGCAATC ATCACACTGTGTCACCTCATTCA | 110Â bp |
sema6d | sema6d-F sema6d-R | GTCTGGTACTCCTTTACAGGGC CGAGGCATGTCCACAAGGAT | 109Â bp |
slc25a42 | slc25a42-F slc25a42-R | ATAAGGGTCATGCCCTACGC GAGGCAGAGTTTTTCCCTGGTA | 100Â bp |
kdf1 | kdf1-F kdf1-R | TCATGAGCAGTGACGTTCCA CTCATCTTCCTCGCCAGTTCA | 73Â bp |