Fig. 6

Expressional regulation analysis of P450s on the local region of the 6th chromosome. A Estimated position of the 12 P450s on chromosome 6; B Position and orientation of 12 P450s on chromosome 6; C Intergenic sequences between two representative groups of P450. D Transcription factor binding region of P450 promoter. Red color indicates DEGs that respond specifically to cyproflanilide; blue color indicates DEGs that respond specifically to chlorantraniliprole; black indicates DEGs responding to all three insecticides; gray indicates no response to any of the three insecticides. ‘– + number’ in Fig. 6B refers to the abbreviations of the accession number of P450 in Fig. 6A, with the last two digits retained for identification. ‘/–/’ refers to the intergenic sequences between two P450 genes. ‘?’ refers to gene loci that could contain multiple P450 genes (also were shown in Supplement table 11). The dashed ellipse indicates the differential binding site for transcription factors in the promoter region. Kr, Krueppel homolog 2 (CG9159 in Drosophila melanogaster genome); Hb, Hunchback (CG9786); Gt, Giant (CG7952), Bcd; Bicoid(CG1034); Adf-1, Adh distal factor-1(CG15845). 1–16 indicates 16 common transcription factors that were shared by the two above upstream sequences, including B factor, Bcd, Dfd, Dl, DSXF, DSXM, En, Eve, Ftz, Mad, Prd, SGF-3, Tll, Zen-1, Zeste [T00918],Zeste [T02100]