Fig. 2

Characterization of seminal plasma exosomes (SPEs) by western blot, nanoparticle tracing analysis (NTA) and transmission electron microscopy (TEM). A Protein expression analysis of Alix, TSG101 and CD81 in seminal plasma exosomes (SPEs), large extracellular vesicles (LEVs) and seminal plasms (SP) samples by western blot. The full size image of western blots can be obtained from Additional file 1: Fig. S1. B Evaluation of size distribution of SPEs determined by NTA analysis. The red dot indicated particles with size of 119.4 nm have the highest density (5.9E+ 6 particles/mL). The main peak of particles size within 20 ~ 200 nm (blue shaded areas) accounted for 99.2% of all particles. C Morphology of isolated SPEs by TEM. The magnification is 10,000× (upper) and 30,000× (lower), respectively