Fig. 3

Identification of differentially expressed lncRNAs involved in regulation of cytochrome P450 (CYP450) and cuticular protein (CP) genes. (a) Venn diagram of showing the number of lncRNAs predicted by four methods, Coding Potential Calculator (CPC, score < 0.5), Coding-Non-Coding Index (CNCI, score < 0), PLEK (score < 0), and Pfam protein structure domain analysis (no annotation). A total of 95 differentially expressed lncRNAs (DELncRNAs) were identified for subsequent tests. (b, d) Co-expression networks between DELncRNA and CYP450 genes, DELncRNA and CP genes using Pearson correlation coefficient. Rectangle and circular nodes indicate target mRNAs (i.e. CYP450 or CP) and DELncRNAs, respectively. Red and blue lines indicate positive and negative correlations, respectively. The lncRNAs with top-ranked degrees are colored red. (c, e) Heatmap of hub lncRNAs ranked by degrees. Data are shown as the average values of expression for each group, and are Z-score normalized across each row. Red bars indicate up-regulated lncRNAs while blue bars indicate down-regulated lncRNAs