Fig. 6

Effects of S100A9 on CPB2-induced apoptosis in IPEC-J2 cells. A S100A9 transfection efficiency assay. B The mRNA levels of Bax and Bcl-2 were detected using RT-qPCR. C The protein levels of Bax and Bcl-2 were detected using western blot analysis. D Mitochondrial membrane potential assay. The enhanced green fluorescence of the JC-1 fluorescent probe indicated increased apoptosis (low mitochondrial membrane potential), and the enhanced red fluorescence indicated that the mitochondrial membrane potential was high. Scale bar = 200 µm. E Hoechst 33,258 staining to detect apoptosis. Scale bar = 200 µm. Blue fluorescence indicates apoptotic cells. *P < 0.05, **P < 0.01