Fig. 12

Phylogenetic analysis of Ct_PGRP_SC-2-like along with other representative PGRP amino acid sequences from vertebrates and invertebrates. Molecular phylogeny was inferred using the maximum-likelihood method in MEGA software (version 11.0). This analysis involved 15 amino acid sequences. The tree was bootstrapped (1,000 replications) and the values are shown along the branches. All ambiguous positions were removed from each sequence pair (pairwise deletion option). The vertebrate and invertebrate taxa are shaded in blue and yellow, respectively. GenBank accession numbers of the analyzed sequences are shown in parentheses. Acc PGRP SC-2, Apis cerana cerana PGRP SC-2 (PBC31638.1); Af PGRP SC-2, Apis florea PGRP SC-2 (XP_003694493.1); Bt PGRP SC-2, Bombus terrestris PGRP SC-2 (XP_012170795.1); Ob PGRP SC-2, Ooceraea biroi PGRP SC-2 (EZA50446.1); At PGRP-2-like, Aethina tumida PGRP-2-like (XP_019877658.1); Tc PGRP SC-2, Tribolium castaneum PGRP SC-2 (XP_008193407.1); Tm PGRP SC-2, Tenebrio molitor PGRP SC-2 (BAJ23047.1); Dm PGRP SC-2, Drosophila melanogaster PGRP SC-2 (CAD89178.1); Lc PGRP SC-2, Lucilia cuprina PGRP SC-2 (KNC21676.1); Tt PGRP SC-2, Triplophysa tibetana PGRP SC-2 (KAA0714663.1); Tf PGRP SC-2, Takifugu flavidus PGRP SC-2 (TWW77559.1); On PGRP SC-2, Oreochromis niloticus PGRP SC-2 (ALM04192.1); Lcc PGRP SC-2, Larimichthys crocea PGRP SC-2 (KAE8295497.1); Lm PGRP SC-2, Lateolabrax maculatus PGRP SC-2 (QQR13803.1)