Fig. 6

Single spore line variant analysis. a Schematic overview of relatedness of all single spore lines. Lines were created by inoculating D. carota root cultures with a single C3 spore. Subsequent generations were made by inoculating a new root culture with a single spore derived from the previous single spore line. Generation (G) number is indicated by color: Black = parental C3 colony, green = G1, blue = G2, red = G3. White circles indicate single spores that were amplified and sequenced, grey circles indicate an established colony producing spores. b-d Allele frequency distributions of several amplified single spores. e MAT loci frequencies of amplified single spores, based on sequencing data. f Principal component analysis of single spores based on allele frequencies of shared SNPs. WGA samples were included as additional control samples. G: Principal component analysis of single spores derived from SS3, including metagenomic DNA from SS3