Fig. 1

(A) Average daily rate of sunflower or wildflower pollen consumption by Bombus impatiens workers either un-infected (circles) or infected with Crithidia bombi (triangles) and submitted for RNA sequencing. Points represent model adjusted means and errors bars 1 SE ± of the mean. These results were used to inform methods for the RNA seq experiment. Only bees that consumed a net positive amount of pollen throughout the 72-h post-inoculation period were selected for RNA sequencing. (B and C) Mean proportion Bombus impatiens workers with detectable Crithidia bombi infection (B) and infection intensity (C). Bees were provided either sunflower pollen (S; orange bars) or control wildflower pollen (W; blue bars) for a period of 24, 48, 72, 96 or 168 h (H) post-inoculation. Bars and error bars represent model adjusted means and one standard error, respectively