Table 5 Information of SSR markers
Primers | F(5’-3’) | R(5’-3’) | Repeat motif | Tm/℃ | Sequence length/bp |
|---|---|---|---|---|---|
M156 | AAGCTAGCAAATACACATAGG | CTGACAAGTTCCAGACAGGG | (CA)14(CT)9N(AAT/C)16 | 56 | 120-152 |
AG28 | TATCGCATCAGGGGTTCCCA | CGGGATGGAGCCGCCAATGA | (GGA)15 | 61 | 222-230 |
M18 | AGGCTCCAAATCCATGCCTC | CAAGAGCAAGAACATAGAATATAA | (AAGA)27 | 52 | 187-195 |
B127 | GAAGGTGCTAACCCTCTTCTGATA | GCTATGGGGTATTGCTAGATGTTT | (TC)27 | 54 | 231-257 |
B004 | TCTGTTACACGTTGCTATTGTACAGG | GATGATGATTCAAACATTCAAACAAAA | (TC)15 | 57 | 216-252 |
B095 | TGGGAAGCAACAGTAATCATAGAG | ACTCTTTTCCCTGTTAAAATTTGC | (TC)14 | 52 | 163-187 |
B041 | ATTCCCTTCCCCATAAGGATCATTC | TACCTAGTGAGGGAGGAAAAGAGA | (CT)14 | 56 | 174-288 |