Table 1 Primers information used in the study
Primers | Sequence (5' to 3') | Application |
|---|---|---|
BCO2-F | CGTGGTGTTTATTGAGCAGCC | qRT-PCR |
BCO2-R | GGCTTTGGCGTGATACTTGACT | qRT-PCR |
β-actin-F | TGGGGCAGTATGGCTTGTATG | qRT-PCR |
β-actin-R | CTCTGGCACCCTAATCACCTCT | qRT-PCR |
BCO2-5′outer | GATTACGCCAAGCTTGGTTTTCTGGCGTTGTCTTTGTCGGA | 5′RACE |
BCO2-3′outer | GATTACGCCAAGCTTCGCTACTTCTACGGTTGCGGTTTCAG | 3′RACE |
BCO2-3′inter | GATTACGCCAAGCTTAAGGTGTGGGAGCATCCTGGACTGTA | 3′RACE |
UPMlong | CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT | 5′RACE/3′RACE |
UPMshort | CTAATACGACTCACTATAGGGC | 5′RACE/3′RACE |
MiR-330 | TTTATTAGCAAAGCACAGGGCCTGC | qRT-PCR |
U6-F | GCTTCGGCAGCACATATACTAAAAT | qRT-PCR |
U6-R | CGCTTCACGAATTTGCGTGTCAT | qRT-PCR |
MiR-330 mimics | GCAAAGCACAGGGCCUGCAGAGA | Overexpression in vitro |
UCUGCAGGCCCUGUGCUUUGCUU | ||
mimics NC | UUCUCCGAACGUGUCACGUTT | Control of miR-330 mimics |
ACGUGACACGUUCGGAGAATT | ||
MiR-330 inhibitor | UCUCUGCAGGCCCUGUGCUUUGC | Downexpression in vitro |
inhibitor NC | CAGUACUUUUGUGUAGUACAA | Control of miR-330 inhibitor |
agomiR-330 | GCAAAGCACAGGGCCUGCAGAGA | Overexpression in vivo |
UCUGCAGGCCCUGUGCUUUGCUU | ||
agomiR-330 NC | UUCUCCGAACGUGUCACGUTT | Control of agomiR-330 |
ACGUGACACGUUCGGAGAATT |