Fig. 2

Phylogenetic tree of ffar2 in vertebrates. FFAR2 protein sequences were aligned using the Maximum Likelihood Method (with Poisson model). Following alignment, the phylogenetic tree was constructed using the neighbour-joining method in Molecular Evolutionary Genetics Analysis (MEGA) software version 7.0 (Tamura 2013). The branch support values were gained by non-parametric bootstrapping (500 replicates). The scale bar represents the calculated evolutionary distance. Genbank accession numbers (from Ensembl or Genoscope databases) are specifed for each species. Mammalian and teleost FFAR2 protein sequences were used to root the tree