Characterization of free fatty acid receptor family in rainbow trout (Oncorhynchus mykiss): towards a better understanding of their involvement in fatty acid signalisation

Table 2 Nucleotide sequence of the PCR primers used to evaluate mRNA expression of FFAR transcripts by RT-qPCR

Transcript	Forward Primer	Reverse Primer	Database	Accession Number
Reference
eef1a1	TCCTCTTGGTCGTTTCGCTG	ACCCGAGGGACATCCTGTG	Ensembl	ENSOMYG00000038328
FFARs
ffar1	ACTGTTGCACCTGAGTCTGG	GCTGGTCCTGGGTGAAGTTC	Ensembl	ENSOMYG00000041396
ffar2a1a	CCGAGTTCCTCTGCTCCATC	TAGGTGATGGGGAAGGCAAC	Ensembl	ENSOMYG00000004986
ffar2a2	GACAACTTCACCCAGGAGCA	AGCAGAACACACAGGCCAG	Ensembl	ENSOMYG00000030315
ffar2b1.1	TTTTCCACACACAGTTGGCC	AGGTAGTGTTGTCGGCATCT	Ensembl	ENSOMYG00000041393
ffar2b1.2	GTGTGGCCTTCCCTATCAGA	GCAGGGCACAATGTACACAA	Ensembl	ENSOMYG00000041387
ffar2b2a	CCCATCCAACACTCGCTGAA	TGATGACGACGATGCTCAGG	Ensembl	ENSOMYG00000030493
ffar2b2b1	TGACCGCAATCAGTGTCGAA	CCCAGAAGAAGACGCTAGCC	Ensembl	ENSOMYG00000030500
ffar2b2b2	GTCCAGTACCATCAACGCCA	CTGCACACTCTCCAACAGGGT	Ensembl	ENSOMYG00000005604

ISSN: 1471-2164