Fig. 1

Transcriptomic profiling of the fetal brain after PS. (A) Mouse model of chronic prenatal stress. C57BL/6slac mice were used to set up timed pregnancy and plugged females were exposed to chronic unexpected mild stress (CUMS) between E5.5 and E14.5 (“Stress” group). Mice without CUMS exposure were used as controls (“Control” group). Whole fetal brains were collected at E15.5 for RNA-seq. (B) Clustered heatmap. N = 9 control/7 stress from 10 litters. (C) MA plot. Red, up-regulated genes; blue, down-regulated genes. Stress vs. Control, Padj < 0.05. (D) qRT-PCR validation in males and females, respectively. N = 6–7/group. Mean ± SEM. *P < 0.05, Unpaired t-test, one-tailed. (E) ShinyGO analysis (“Biological Process”) of up- and down-regulated genes. The size of dot indicates the number of genes in the enriched pathway. Enrichment score calculated as -Log10FDR.