Fig. 2

Hybridization of probes for scaffold mapping. a Hybridization of a group of ten scaffold probe sets, the first step for scaffold anchoring. Scaffold probes were labeled with FAM (cyan), ATTO-550 (yellow), ATTO-647 (red) or with a pair to generate another color (green for FAM + ATTO-550, orange for ATTO-550 + ATTO-647, violet for FAM + ATTO-647). b Example of unclear or mis-assembled signal scaffolds confirmed by hybridization in a smaller group. c Whole karyotype covered by the 68 scaffold probe sets allowing identification of all chromosomes with a single hybridization. All scaffolds were assigned to one pair of chromosomes by identifying their color scheme. Scale bar = 5 μm