Fig. 2
From: Highly accurate long reads are crucial for realizing the potential of biodiversity genomics
A case study comparing the capacity for two long-read sequencing technologies to assemble the complex gene underlying silk production in caddisflies, H-fibroin. a Raw Oxford Nanopore (ONT) reads mapped to the consensus H-fibroin sequence from the ONT assembly. b Raw HiFi reads mapped to the primary H-fibroin sequence from the phased HiFi assembly. Dark lines indicate mismatches relative to consensus. In (b), mismatches reflect an H-fibroin length polymorphism that can be resolved by subsampling reads based on their allele-specificity. Note the high amount of noise within reads relative to the consensus for highly accurate long reads (b) versus longer, but less accurate, reads (a)