Fig. 3

Phylogenetic tree was constructed based on neighbor-joining using MEGA 11.0.11 version software with 100 bootstrap replications. The value of bootstrap replicates is shown at the nodes and blue marked as an outgroup. a. Phylogenetic tree of assimilatory nitrate reductase; JFLDCCAL 02528- an isolated L. amnigena strain similar to the top three NCBI strains whose accession no. WP239609066, WP204771324, WP202666067; Bacillus subtilis (WP015382725) is used as out group reference (blue marked). Bootstrap value is indicated on nodes (in bold), branch length value below the node, and scale bar value indicates 0.20 substitutions per nucleotide position. b. Phylogenetic tree of respiratory nitrate reductase; JFLDCCAL02523 nitrate reductase- an isolated L. amnigena strain is similar to top three NCBI strain whose accession numbers are WP239609064, WP202667856, WP 131487214; Bacillus strain (WP003243085) is used as an out group reference (blue marked). Bootstrap value is indicated on nodes (in bold), branch length value below the node and scale bar value indicates 0.050 substitutions per nucleotide position. c. Phylogenetic tree of nitrite reductase; JFLDCCAL02529 nitrite reductase- an isolated L. amnigena strain is similar to top three accession numbers WP015960754, WP202714325 and WP202694693; Bacillus strain (WP003234637) is used as an outgroup reference (blue marked). Bootstrap value is indicated on nodes (in bold), branch length value below the node and scale bar value indicates 0.10 substitutions per nucleotide position. d. Phylogenetic tree of nitric oxide reductase; JFLDCCAL02959-an isolated L. amnigena strain similar to top three strains whose accession no. WP239609353, WP202667624, WP216981010; Pseudomonas sp. (OCX92857) is used as an outgroup reference (blue marked). Bootstrap value is indicated on nodes (in bold), branch length value below the node and scale bar value indicates 0.20 substitutions per nucleotide position. e. Phylogenetic tree of hydroxylamine reductase; JFLDCCAL 00570- an isolated L. amnigena strain is similar to top three strains accession no. WP204772478, WP219346828 and WP131488445; Rhodobacter capsulatus strain is used as an outgroup reference (blue marked). Bootstrap value is indicated on nodes (in bold), branch length value below the node and scale bar value indicates 0.050 substitutions per nucleotide position. f. Phylogenetic tree of glutamine synthetase, JFLDCCAL03269- an isolated L. amnigena strain similar to top three strains WP015961054, WP062776483, WP095284040; Bacillus strain is used as an outgroup reference (blue marked). Bootstrap value is indicated on nodes (in bold), branch length value below the node and scale bar value indicates 0.10 substitutions per nucleotide position