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Fig. 4 | BMC Genomics

Fig. 4

From: An SPRI beads-based DNA purification strategy for flexibility and cost-effectiveness

Fig. 4

Schematic overview of the library preparation and data analysis. A Experimental workflow of the library preparation procedure using the Control, SDPS and ASDPS. B Read depth correlation shows consistently high coverage among these libraries of the Control, SDPS and ASDPS. Coverage of each 10 kb region of mm9 (as determined by bedtools coverage) was compared among Control, SDPS and ASDPS. Most regions are covered by ~ 1000 reads, as expected. Low and high coverage regions are well correlated. Pearson correlation values among each pair of comparisons, with the size of the text proportional to the magnitude of the correlation coefficient (upper diagonal elements). C Histogram of Reads distribution in different regions of the genome. Each square bar in the figure represents a library, the height of each region represents the percentage of Mapped Reads in all Mapped Reads Mapped to this region. D GC coverage of the six DNA libraries. Expected normalized coverage of 1.0 is indicated by the horizontal grey line, the number of 100 bp regions at each GC% is indicated by the vertical grey bars, and the colored lines represent the normalized coverage for each library

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