Fig. 3

Discrepancy in DEGs called between RNA-seq and Iso-Seq. A Average expression of RNA-seq (counts) vs both Iso-Seq + RNA-seq (counts) (B) Average expression of Iso-Seq vs both Iso-Seq + RNA-seq (counts) (C) Multi-mapping level of all DEGs across three different categories grouped by analysis methods for each sample. For each DEG per sample, the level of multi-mapping was defined as: (the number of multi-mapped reads / total number of mapped reads) × 100%. D Overlapping of genomic coordinates of all DEGs across three different categories grouped by analysis methods. For each DEG, the percentage of overlap with other genes was calculated as: (length of overlapped positions with other genes / length of DEG) × 100%