Table 1 Mapping statistics of RNA-seq and Iso-Seq. The % of mapped reads and assigned genes were calculated based on cleaned reads
From: Long read isoform sequencing reveals hidden transcriptional complexity between cattle subspecies
Sample | RNA-seq raw reads | RNA-seq cleaned reads | RNA-seq mapped reads (%) | RNA-seq assigned to genes (%) | Iso-seq CCS reads | Iso-seq FLNC reads |
|---|---|---|---|---|---|---|
Angus_7 | 49,189,375 | 47,752,086 | 95.66% | 75.70% | 8,840,157 | 8,124,187 |
Angus_53 | 51,315,712 | 50,634,082 | 94.88% | 76.30% | 8,730,907 | 7,893,212 |
Angus_60 | 103,506,419 | 101,100,269 | 95.97% | 76.80% | 8,388,042 | 7,759,609 |
Brahman_65 | 45,816,738 | 44,996,438 | 95.26% | 76.90% | 8,578,336 | 7,631,806 |
Brahman_22 | 53,889,070 | 53,018,406 | 94.99% | 76.30% | 10,983,128 | 9,340,386 |
Brahman_99 | 57,939,684 | 56,934,361 | 95.94% | 77.60% | 10,217,439 | 8,404,511 |