Fig. 1
Effect of green feed on transcriptional profile in rumen of dairy buffaloes (six animals for each condition), identified using EdgeR (nominal p-values < 0.05). a Top: Volcano plot that illustrates differentially expressed genes (DEGs) in rumen of dairy buffaloes fed a TMR + green feed (Treated) versus animals fed the TMR diet (Control). The significance of the differential expression (−log10 (p-value), y-axis) is plotted versus fold change expression level (x-axis). Red and green dots depict the genes that are significantly up- and down-regulated in treated versus control animals; black dots are genes whose expression levels did not reach the statistical significance (p-value < 0.05) between the two experimental groups. Bottom: Bar graph that shows the number of up- and down-regulated genes in treated vs control animals. b Heatmap representation of significant DEGs (155) across all the samples. Blue-red gradient shows low to high expression levels for each gene. Dendrogram represents a hierarchical clustering based on Pearson correlation values