Table 5 List of representative differentially expressed transcripts and comparison of fold changes in gene expression determined by RT-qPCR, with RNA-Seq data
Gene name | RNA seq | qRT PCR | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Erianthus arundinaceus | Saccharum spontaneum | Co 86032 | Erianthus arundinaceus | Saccharum spontaneum | Co 86032 | |||||||
Log2 fold change | P Value | Log2 fold change | P Value | Log2 fold change | P Value | Log2 fold change | P Value | Log2 fold change | P Value | Log2 fold change | P Value | |
Dehydration responsive gene | 21.64 | 0.00 | -0.84 | 0.00 | 3.41 | 0.00 | 3.11 | 0.00 | -1.63 | 0.00 | 3.23 | 0.01 |
Cellulose synthase (EC 2.4.1.12) | 1.45 | 0.00 | 22.61 | 0.00 | 2.05 | 0.00 | 1.23 | 0.00 | 3.43 | 0.01 | 2.13 | 0.01 |
Calmodulin binding protein | 0.55 | 0.01 | 17.74 | 0.00 | 2.26 | 0.00 | 1.42 | 0.01 | 2.97 | 0.00 | 1.40 | 0.01 |
Cytochrome P450 | 5.14 | 0.01 | 10.10 | 0.00 | -0.68 | 0.00 | 2.21 | 0.01 | 3.56 | 0.00 | -1.34 | 0.01 |
Posphohexokinas (EC 2.7.1.11) | -0.93 | 0.01 | -0.83 | 0.00 | -0.44 | 0.01 | -1.24 | 0.01 | -2.1 | 0.01 | -2.65 | 0.00 |
Peroxidase (EC 1.11.1.7) | -0.78 | 0.00 | -0.96 | 0.0 | -0.66 | 0.0 | -1.45 | 0.00 | -1.09 | 0.00 | -1.12 | 0.00 |
Defensin | 5.31 | 0.00 | -0.39 | 0.0 | -0.91 | 0.0 | 1.54 | 0.00 | -2.34 | 0.00 | -3.20 | 0.00 |
Ammonium transporter | -0.87 | 0.00 | -0.65 | 0.0 | -0.80 | 0.0 | -2.43 | 0.00 | -4.60 | 0.01 | -0.30 | 0.0 |