Table 1 ‘N’ spacer-linked primer pool combination
‘N’ spacer primer pool combinations | Forward Primer Pool | Reverse Primer Pool |
|---|---|---|
6 N Spacer primer pool | 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N | 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N |
7 N Spacer primer pool | 7 N, 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N | 7 N, 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N |
8 N Spacer primer pool | 8 N, 7 N, 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N | 8 N, 7 N, 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N |
9 N Spacer primer pool | 9 N, 8 N, 7 N, 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N | 9 N, 8 N, 7 N, 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N |
10 N Spacer primer pool | 10 N, 8 N, 7 N, 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N | 10 N, 8 N, 7 N, 6 N, 5 N, 4 N, 3 N, 2 N, 1 N, 0 N |