Fig. 5
From: Genome-wide identification of Kanamycin B binding RNA in Escherichia coli
SHAPE probing of ibsC mRNA on Kanamycin B titration. a Electropherogram of NMIA-modified RNA with NMIA ([ +] NMIA, blue trace), compared with that of unmodified RNA ([ −] NMIA, black trace). b Analysis of NMIA modification electropherogram of ibsC RNA with 20 μM Kanamycin B (red trace), compared with 0 μM Kanamycin B (blue trace). c The secondary structure of the ibsC RNA [53]. d Histogram of NMIA modification of the in vitro transcribed RNA nucleotides (58–62) on titration of Kanamycin B. The reactivity of the nucleotide is calculated as the ratio of the height of the nucleotide to the height of all nucleotides. e Kanamycin B binding measured through changes in NMIA reactivity at the 107U position in the RNA, on titration with Kanamycin B (data taken from Figure S2)