Fig. 3

Dynamic spermatogenic gene expression patterns and cell-type specific gene markers in pubertal germ cells of cattle-yak and yak. A, B Developmental pseudotime of pubertal cattle-yak and yak germ cells, respectively. Cell types were identified from the clusters in corresponding tSNE plots and could be distinguished with different colors. Arrows show the developmental order of the germ cells. C, D Dynamic expression of selected germ cell signature genes during pubertal cattle-yak and yak spermatogenesis, respectively. The cell types were marked with different color and the cell numbers were showed with different length of bars. The red gradients from dark to light indicate high to low expression level. E, F Expression patterns of selected germ cell marker genes on tSNE plots of cattle-yak and yak, respectively. The blue gradients from dark to light indicate high to low expression levels