Fig. 5

Pangenomic analysis. A Whole genome phylogenetic analysis of P. pacificasedimenti and P. pacificagyrus conducted using concatenated single-copy marker genes determined by GET_HOMOLOGUES [36] which used the OrthoMCL algorithm. The tree was estimated using maximum likelihood analysis and 1,000 bootstraps in IQ-TREE [37]. Bootstrap support values are shown at each node. The best-fit model chosen by ModelFinder [38] was GTR2 + FO + ASC + G4. The tree was re-rooted with Aspergillus species as the outgroup and visualized with iTOL [39]. B Venn diagram generated with OrthoVenn2 [40] showing the number of shared and unique protein clusters between our isolates and their closest relative. C Summary of the number of proteins, protein clusters, and single proteins within each genome