Fig. 5

(a) Western blot analysis of PRDX6 abundance in GFE and PFE sperms. The samples derived from the same experiment and all blots were processed in parallel. The bars represented the mean values ± SEM of the intensity of all the bands per lane expressed in arbitrary units (A.U.) using sperm in three independent experiments. (b) The effect of PRDX6 (0.0, 0.01, 0.1 and 1 mg/L) on the frozen-thawed sperm motility of Mediterranean buffaloes. (c) The effect of PRDX6 on the ROS levels of frozen-thawed sperm. (d) The effect of PRDX6 on the DNA oxidation of frozen-thawed sperm. (e) The effect of PRDX6 on the lipid oxidation of frozen-thawed sperm. (f) The effect of PRDX6 on the total antioxidant capacity of frozen-thawed sperm. The t test was used to compare data between two groups and variance (ANOVA) was used to compare data between multiple groups. *P < 0.05, **P < 0.01