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Table 4 Confirmation of 6 DEPs as detected during iTRAQ analysis by using PRM analysis

From: iTRAQ-based comparative proteomics reveal an enhancing role of PRDX6 in the freezability of Mediterranean buffalo sperm

Protein ID

Description (NCBI)

Fold change

(iTRAQ)

Fold change

(PRM)

P15690

NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

0.649384656

0.749665923

Q2KJE5

Glyceraldehyde-3-phosphate dehydrogenase, testis-specific

0.577890724

0.722641837

E1BD64

Mitochondrial pyruvate carrier

0.367672664

0.684912461

F1MYH5

A-kinase anchoring protein 4

0.56593542

0.632369262

F1N2F2

Phosphoglycerate mutase

1.590575011

1.899681405

Q24JZ7

Succinyl-CoA:3-ketoacid-coenzyme A transferase

0.608101513

0.603642301