Fig. 1

Characterization of different group genes with promoter CGIs in COAD. A Left panel shows the DNA methylation difference of COAD over normal colon tissue. Middle and right panels show DNA methylation patterns at promoter CGIs of normal colon tissue and COAD specifically. Each line represents a single CGI. B Upset plot showing the overlap of all-hyper group genes in different cancer types. C Upset plot showing the overlap of C3 group genes in different cancer types. D The box plot showing the abundance of genes from 5 groups in normal colon tissue or COAD. TPM: transcripts per million. Data were presented as mean ± SD. Statistical analysis was performed by Wilcoxon Rank Sum, ****p < 0.0001. E The stacked bar plot showing the distribution of promoter CGIs in 5 groups in PMDs, HMDs and other regions in COAD. PMDs: partially methylated domains, HMDs: highly methylated domains. F The stacked bar plot showing proportion of bivalent and non-bivalent genes in 5 groups in COAD. G The box plot showing Tau index of genes in 5 groups in COAD. Data were presented as mean ± SD. Statistical analysis was performed by Wilcoxon Rank Sum, **** p < 0.0001. H Tissue enrichment of genes in C3, C4 and C5 groups in COAD. I Heat map showing GO analysis of genes involved in different groups in COAD