Fig. 5
LSD1 regulates the proliferation and migration of HCT116 cells via OVOL2. A qPCR results showing the OVOL2 gene expression level in WT and LSD1 KO HCT116 cells. The experiments performed with three biological replicates. Data were presented as mean ± SD. Statistical analysis was performed by Student’s t test, **p < 0.01. B Integrative Genomics Viewer (IGV) browser track depicting distribution pattern and levels of DNA methylation at OVOL2 promoter in normal colon tissue, WT and LSD1 KO HCT116 cells, as well as control and LSD1 OE NCM460 cells (top 5 panels); distribution pattern and levels of H3K4me1/3 and H3K27me3 at OVOL2 promoter in WT and LSD1 KO HCT116 cells (lower 6 panels); CGIs were highlighted by green bars, DNAm: DNA methylation. C ChIP-qPCR of LSD1 at OVOL2 promoter and a control region in HCT116 cells. TSS was calculated as 0. IgG was used as a control for LSD1 antibody. control region: -4359/-4158 bp. The experiments performed with three biological replicates. Data were presented as mean ± SD. Statistical analysis was performed by Student’s t test, ***p < 0.001. D Wound healing assay results showed the migration of indicated cells at 0 h and 48 h (left panel). Quantification of the images was plotted in the right panel. Scale bar, 100 µm. The experiments were performed with three biological replicates. Data were presented as mean ± SD. Statistical analysis was performed by Student’s t test, ns: not significantly, * p < 0.05, ** p < 0.01. E Left panels show the representative images of transwell assay of indicated cells. Scale bar, 100 µm. Quantification of the images was plotted in the right panel. The experiments were performed with three biological replicates. Data were presented as mean ± SD. Statistical analysis was performed by Student’s t test, * p < 0.05, ** p < 0.01. F Left panels show the representative images of colony formation assay of the indicated cells. Quantification of the images was plotted in the right panel. The experiments were performed with three biological replicates. Data were presented as mean ± SD. Statistical analysis was performed by Student’s t test, * p < 0.05, *** p < 0.001