Fig. 4

The first Huntiella a-factor pheromone locus, harboring four genes in the five heterothallic species and only three genes in the three unisexual species. These pheromone genes are flanked by genes encoding a tubulin α chain and a nucleolar protein. A A synteny map of this locus across all eight species considered, showing high levels of similarity within the heterothallic species and within the unisexual species. Significant regions of the heterothallic loci are missing from the unisexual loci, including one of the a-factor pheromone homologs. This figure was generated using EasyFig V2.2.2. B Protein sequences of the homologs from selected heterothallic and unisexual representatives. This shows the modular nature of these proteins, with the CpaX domain indicated in blue text and stop codons indicated by an asterisk. The a4 gene is likely the only homolog that produces a full length and functional protein in H. moniliformis