Table 1 The information of the top 10 candidate reference genes in the early development, normal adult tissues and hindgut under sulfide stress of the U. unicinctus
| Â | Gene ID | Gene name | CV | Mean |
|---|---|---|---|---|
Early development | evm.model.Hic_asm_9.985.3 | FAD-dependent oxidoreductase domain-containing protein 1, FXRD1 | 0.0157 | 14.329 |
evm.model.Hic_asm_2.658 | Caprin-1, CAPR1 | 0.0170 | 14.326 | |
evm.model.Hic_asm_11.431 | Putative neutral sphinGOmyelinase, NSMA | 0.0171 | 14.326 | |
evm.model.Hic_asm_13.131 | Translation initiation factor IF-2 Mitochondrial, IF2M | 0.0183 | 14.323 | |
evm.model.Hic_asm_2.533 | Syntaxin-12, STX12 | 0.0190 | 14.322 | |
evm.model.Hic_asm_6.652 | Programmed cell death protein 6, PDCD6 | 0.0191 | 14.326 | |
evm.model.Hic_asm_3.310.1 | Pentatricopeptide repeat domain-containing protein 3, mitochondrial, PTCD3 | 0.0192 | 14.320 | |
evm.model.Hic_asm_6.1042 | Transcription factor 25, TCF25 | 0.0201 | 14.319 | |
evm.model.Hic_asm_11.1625 | TRK-fused gene protein, TFG | 0.0202 | 14.319 | |
evm.model.Hic_asm_0.1156 | HBS1-like protein, HBS1L | 0.0203 | 14.317 | |
Normal adult tissues | evm.model.Scaf262.4 | Ubiquitin conjugation factor E4 A, UBE4A | 0.0093 | 15.532 |
evm.model.Hic_asm_10.1197 | UDP-N-acetylglucosamine–peptide N-acetylglucosaminyltransferase 110 kDa subunit, OGT1 | 0.0102 | 15.531 | |
novel.9221 | Not annotated | 0.0114 | 15.528 | |
evm.model.Hic_asm_9.1113_evm.model.Hic_asm_9.1114 | Histone-lysine N-methyltransferase, EHMT1 | 0.0114 | 15.528 | |
evm.model.Hic_asm_8.1166 | ADP-ribosylation factor-binding protein, GGA3 | 0.0118 | 15.528 | |
evm.model.Hic_asm_7.291 | Transformer-2 protein homolog beta, TRA2B | 0.0125 | 15.526 | |
evm.model.Hic_asm_4.469 | F-box only protein 18, FBX18 | 0.0129 | 15.525 | |
evm.model.Hic_asm_3.391 | Uncharacterized protein STK_23830 | 0.0131 | 15.525 | |
evm.model.Hic_asm_13.300 | Pre-mRNA-processing factor 39, PRP39 | 0.0134 | 15.524 | |
evm.model.Scaf156.13 | Rabenosyn-5, RBNS5 | 0.0138 | 15.524 | |
Hindgut under sulfide stress | evm.model.Hic_asm_6.1089 | CCR4-NOT transcription complex subunit 1, CNOT1 | 0.0018 | 16.346 |
evm.model.Hic_asm_1.1227 | Clathrin heavy chain 1, CLH1 | 0.0027 | 16.346 | |
evm.model.Hic_asm_3.989 | Exocyst complex component 6, EXOC6 | 0.0027 | 16.346 | |
evm.model.Hic_asm_14.1611 | Lysosomal alpha-glucosidase, LYAG | 0.0027 | 16.346 | |
evm.model.Hic_asm_7.654 | Not annotated | 0.0028 | 16.346 | |
evm.model.Hic_asm_3.766 | Melanotransferrin, TRFM | 0.0030 | 16.346 | |
evm.model.Hic_asm_16.105 | Angiomotin, AMOT | 0.0031 | 16.346 | |
evm.model.Hic_asm_10.1107 | RNA-binding protein 26, RBM26 | 0.0031 | 16.346 | |
evm.model.Hic_asm_2.1309 | Calumenin-B, CALUB | 0.0031 | 16.346 | |
evm.model.Hic_asm_13.2016 | HEAT repeat-containing protein 5B, HTR5B | 0.0032 | 16.346 |