Articles

STRP Screening Sets for the human genome at 5 cM density

Short tandem repeat polymorphisms (STRPs) are powerful tools for gene mapping and other applications. A STRP genome scan of 10 cM is usually adequate for mapping single gene disorders. However mapping studies ...

Authors: Nader Ghebranious, David Vaske, Adong Yu, Chengfeng Zhao, Gabor Marth and James L Weber

Ancient conserved domains shared by animal soluble guanylyl cyclases and bacterial signaling proteins

Soluble guanylyl cyclases (SGCs) are dimeric enzymes that transduce signals downstream of nitric oxide (NO) in animals. They sense NO by means of a heme moiety that is bound to their N-terminal extensions.

Authors: Lakshminarayan M Iyer, Vivek Anantharaman and L Aravind

Reproducibility of oligonucleotide arrays using small samples

Low RNA yields from small tissue samples can limit the use of oligonucleotide microarrays (Affymetrix GeneChips^®). Methods using less cRNA for hybridization or amplifying the cRNA have been reported to reduce the...

Authors: Jeanette N McClintick, Ronald E Jerome, Charles R Nicholson, David W Crabb and Howard J Edenberg

In silico characterisation and chromosomal localisation of human RRH (peropsin) – implications for opsin evolution

The vertebrate opsins are proteins which utilise a retinaldehyde chromophore in their photosensory or photoisomerase roles in the visual/irradiance detection cycle. The majority of the opsins, such as rod and ...

Authors: James Bellingham, Dominic J Wells and Russell G Foster

Gene trap mutagenesis of hnRNP A2/B1: a cryptic 3' splice site in the neomycin resistance gene allows continued expression of the disrupted cellular gene

Tagged sequence mutagenesis is a process for constructing libraries of sequenced insertion mutations in embryonic stem cells that can be transmitted into the mouse germline. To better predict the functional co...

Authors: Michael Roshon, James V DeGregori and H Earl Ruley

Comparative study of methyl-CpG-binding domain proteins

Methylation at CpG dinucleotides in genomic DNA is a fundamental epigenetic mechanism of gene expression control in vertebrates. Proteins with a methyl-CpG-binding domain (MBD) can bind to single methylated Cp...

Authors: Tim C Roloff, H Hilger Ropers and Ulrike A Nuber

Cynomolgus monkey testicular cDNAs for discovery of novel human genes in the human genome sequence

In order to contribute to the establishment of a complete map of transcribed regions of the human genome, we constructed a testicular cDNA library for the cynomolgus monkey, and attempted to find novel transcr...

Authors: Naoki Osada, Munetomo Hida, Jun Kusuda, Reiko Tanuma, Makoto Hirata, Yumiko Suto, Momoki Hirai, Keiji Terao, Sumio Sugano and Katsuyuki Hashimoto

Outliers involving the Poly(A) effect among highly-expressed genes in microarrays

The Poly(A) effect is a cross-hybridization artifact in which poly(T)-containing molecules, which are produced by the reverse transcription of a poly(A)⁺ RNA mixture, bind promiscuously to the poly(A) stretches o...

Authors: Shujia J Pan, David R Rigney and John L Ivy

DNA sequence conservation between the Bacillus anthracis pXO2 plasmid and genomic sequence from closely related bacteria

Complete sequencing and annotation of the 96.2 kb Bacillus anthracis plasmid, pXO2, predicted 85 open reading frames (ORFs). Bacillus cereus and Bacillus thuringiensis isolates that ranged in genomic similarity t...

Authors: James Pannucci, Richard T Okinaka, Erin Williams, Robert Sabin, Lawrence O Ticknor and Cheryl R Kuske

The catalytic domains of thiamine triphosphatase and CyaB-like adenylyl cyclase define a novel superfamily of domains that bind organic phosphates

The CyaB protein from Aeromonas hydrophila has been shown to possess adenylyl cyclase activity. While orthologs of this enzyme have been found in some bacteria and archaea, it shows no detectable relationship to ...

Authors: Lakshminarayan M Iyer and L Aravind

Domain-oriented functional analysis based on expression profiling

Co-regulation of genes may imply involvement in similar biological processes or related function. Many clusters of co-regulated genes have been identified using microarray experiments. In this study, we examin...

Authors: Wei Ding, Luquan Wang, Ping Qiu, Mitchel Kostich, Jonathan Greene and Marco Hernandez

Optimization and evaluation of T7 based RNA linear amplification protocols for cDNA microarray analysis

T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different ampl...

Authors: Hongjuan Zhao, Trevor Hastie, Michael L Whitfield, Anne-Lise Børresen-Dale and Stefanie S Jeffrey

Mutation screening of two candidate genes from 13q32 in families affected with Bipolar disorder: human peptide transporter (SLC15A1) and human glypican5 (GPC5)

Multiple candidate regions as sites for Schizophrenia and Bipolar susceptibility genes have been reported, suggesting heterogeneity of susceptibility genes or oligogenic inheritance. Linkage analysis has sugge...

Authors: Manjula Maheshwari, SL Christian, C Liu, JA Badner, S Detera-Wadleigh, ES Gershon and Richard A Gibbs

A reference database for tumor-related genes co-expressed with interleukin-8 using genome-scale in silico analysis

The EST database provides a rich resource for gene discovery and in silico expression analysis. We report a novel computational approach to identify co-expressed genes using EST database, and its application to I...

Authors: Lawrence Benbow, Lynn Wang, Maureen Laverty, Suxing Liu, Ping Qiu, Richard W Bond, Eric Gustafson, Joseph A Hedrick, Mitchell Kostich, Jonathan R Greene and Luquan Wang

Assessment of differential gene expression in human peripheral nerve injury

Microarray technology is a powerful methodology for identifying differentially expressed genes. However, when thousands of genes in a microarray data set are evaluated simultaneously by fold changes and signif...

Authors: Yuanyuan Xiao, Mark R Segal, Douglas Rabert, Andrew H Ahn, Praveen Anand, Lakshmi Sangameswaran, Donglei Hu and C Anthony Hunt

Cross-species hybridisation of pig RNA to human nylon microarrays

The objective of this research was to investigate the reproducibility of cross-species microarray hybridisation. Comparisons between same- and cross-species hybridisations were also made. Nine hybridisations b...

Authors: DE Moody, Z Zou and L McIntyre

Mouse ribonuclease III. cDNA structure, expression analysis, and chromosomal location

Members of the ribonuclease III superfamily of double-stranded(ds)-RNA-specific endoribonucleases participate in diverse RNA maturation and decay pathways in eukaryotic and prokaryotic cells. A human RNase III...

Authors: Kristine R Fortin, Rhonda H Nicholson and Allen W Nicholson

In silico and in situ characterization of the zebrafish (Danio rerio) gnrh3 (sGnRH) gene

Gonadotropin releasing hormone (GnRH) is responsible for stimulation of gonadotropic hormone (GtH) in the hypothalamus-pituitary-gonadal axis (HPG). The regulatory mechanisms responsible for brain specificity ...

Authors: Jacob Torgersen, Rasoul Nourizadeh-Lillabadi, Harald Husebye and Peter Aleström

High-resolution physical map for chromosome 16q12.1-q13, the Blau syndrome locus

The Blau syndrome (MIM 186580), an autosomal dominant granulomatous disease, was previously mapped to chromosome 16p12-q21. However, inconsistent physical maps of the region and consequently an unknown order o...

Authors: Xiaoju Wang, Helena Kuivaniemi, Gina Bonavita, Charlene J Williams and Gerard Tromp

Pervasive properties of the genomic signature

The dinucleotide relative abundance profile can be regarded as a genomic signature because, despite diversity between species, it varies little between 50 kilobase or longer windows on a given genome. Both the...

Authors: Robert W Jernigan and Robert H Baran

Multigene family isoform profiling from blood cell lineages

Analysis of cell-selective gene expression for families of proteins of therapeutic interest is crucial when deducing the influence of genes upon complex traits and disease susceptibility. Presently, there is n...

Authors: Grant Dewson, Edward C Conley and Peter Bradding

Comparative analysis of somitogenesis related genes of the hairy/Enhancer of split class in Fugu and zebrafish

Members of a class of bHLH transcription factors, namely the hairy (h), Enhancer of split (E(spl)) and hairy-related with YRPW motif (hey) (h/E(spl)/hey) genes are involved in vertebrate somitogenesis and some of...

Authors: Martin Gajewski and Chris Voolstra

"In-gel" purified ditags direct synthesis of highly efficient SAGE Libraries

SAGE (serial analysis of gene expression) is a recently developed technique for systematic analysis of eukaryotic transcriptomes. The most critical step in the SAGE method is large scale amplification of ditag...

Authors: Saroj P Mathupala and Andrew E Sloan

Defining signal thresholds in DNA microarrays: exemplary application for invasive cancer

Genome-wide or application-targeted microarrays containing a subset of genes of interest have become widely used as a research tool with the prospect of diagnostic application. Intrinsic variability of microar...

Authors: M Bilban, LK Buehler, S Head, G Desoye and V Quaranta

Expression and genomic analysis of midasin, a novel and highly conserved AAA protein distantly related to dynein

The largest open reading frame in the Saccharomyces genome encodes midasin (MDN1p, YLR106p), an AAA ATPase of 560 kDa that is essential for cell viability. Orthologs of midasin have been identified in the geno...

Authors: Joan E Garbarino and I R Gibbons

GPR99, a new G protein-coupled receptor with homology to a new subgroup of nucleotide receptors

Based on sequence similarity, the superfamily of G protein-coupled receptors (GPRs) can be subdivided into several subfamilies, the members of which often share similar ligands. The sequence data provided by t...

Authors: Timo Wittenberger, Susanne Hellebrand, Antonia Munck, Hans-Jürgen Kreienkamp, H Chica Schaller and Wolfgang Hampe

Obtaining reliable information from minute amounts of RNA using cDNA microarrays

High density cDNA microarray technology provides a powerful tool to survey the activity of thousands of genes in normal and diseased cells, which helps us both to understand the molecular basis of the disease ...

Authors: Limei Hu, Jing Wang, Keith Baggerly, Hua Wang, Gregory N Fuller, Stanley R Hamilton, Kevin R Coombes and Wei Zhang

Complete genome sequence of a novel extrachromosomal virus-like element identified in planarian Girardia tigrina

Freshwater planarians are widely used as models for investigation of pattern formation and studies on genetic variation in populations. Despite extensive information on the biology and genetics of planaria, th...

Authors: Denis V Rebrikov, Maria E Bulina, Ekaterina A Bogdanova, Loura L Vagner and Sergey A Lukyanov

Erratum to: The cloning, genomic organization and tissue expression profile of the human DLG5 gene: Correction

Authors: Gopi Shah, Ramon Brugada, Oscar Gonzalez, Grazyna Czernuszewicz, Richard A Gibbs, Linda Bachinski and Robert Roberts

Complex splicing pattern generates great diversity in human NF1 transcripts

Mutation analysis of the neurofibromatosis type 1 (NF1) gene has shown that about 30% of NF1 patients carry a splice mutation resulting in the production of one or several shortened transcripts. Some of these tra...

Authors: Ina Vandenbroucke, Tom Callens, Anne De Paepe and Ludwine Messiaen

Efficacy of SSH PCR in isolating differentially expressed genes

Suppression Subtractive Hybridization PCR (SSH PCR) is a sophisticated cDNA subtraction method to enrich and isolate differentially expressed genes. Despite its popularity, the method has not been thoroughly s...

Authors: Wan Ji, Matthew B Wright, Li Cai, Angel Flament and Klaus Lindpaintner

Molecular cloning and sequence analysis of hamster CENP-A cDNA

The centromere is a specialized locus that mediates chromosome movement during mitosis and meiosis. This chromosomal domain comprises a uniquely packaged form of heterochromatin that acts as a nucleus for the ...

Authors: Javier Figueroa, Carlos Pendón and Manuel M Valdivia

Analysis of a human brain transcriptome map

Genome wide transcriptome maps can provide tools to identify candidate genes that are over-expressed or silenced in certain disease tissue and increase our understanding of the structure and organization of th...

Authors: Ping Qiu, Lawrence Benbow, Suxing Liu, Jonathan R Greene and Luquan Wang

Four signature motifs define the first class of structurally related large coiled-coil proteins in plants.

Animal and yeast proteins containing long coiled-coil domains are involved in attaching other proteins to the large, solid-state components of the cell. One subgroup of long coiled-coil proteins are the nuclea...

Authors: Frank Gindullis, Annkatrin Rose, Shalaka Patel and Iris Meier

Classification and evolutionary history of the single-strand annealing proteins, RecT, Redβ, ERF and RAD52

The DNA single-strand annealing proteins (SSAPs), such as RecT, Redβ, ERF and Rad52, function in RecA-dependent and RecA-independent DNA recombination pathways. Recently, they have been shown to form similar h...

Authors: Lakshminarayan M Iyer, Eugene V Koonin and L Aravind

Digital analysis of cDNA abundance; expression profiling by means of restriction fragment fingerprinting

Gene expression profiling among different tissues is of paramount interest in various areas of biomedical research. We have developed a novel method (DADA, D igital A nalysis of cD NA A bundance), that calculates...

Authors: Peter Hof, Claudia Ortmeier, Kirstin Pape, Birgit Reitmaier, Johannes Regenbogen, Andreas Goppelt and Joern-Peter Halle

The cloning, genomic organization and tissue expression profile of the human DLG5 gene

Familial atrial fibrillation, an autosomal dominant disease, was previously mapped to chromosome 10q22. One of the genes mapped to the 10q22 region is DLG5, a member of the MAGUKs (Membrane Associated Gyanylat...

Authors: Gopi Shah, Ramon Brugada, Oscar Gonzalez, Grazyna Czernuszewicz, Richard A Gibbs, Linda Bachinski and Robert Roberts

Cloning and characterization of the rat homologues of the Inhibitor of Apoptosis protein 1, 2, and 3 genes.

Inhibitor of Apoptosis (IAP) proteins are key intrinsic regulators of apoptosis induced by a variety of triggers. We isolated the rat Inhibitor of Apoptosis genes 1, 2 and 3 and characterized their tissue dist...

Authors: Martin Holcik, Charles A Lefebvre, Keiko Hicks and Robert G Korneluk

Bootstrap, Bayesian probability and maximum likelihood mapping: exploring new tools for comparative genome analyses

Horizontal gene transfer (HGT) played an important role in shaping microbial genomes. In addition to genes under sporadic selection, HGT also affects housekeeping genes and those involved in information proces...

Authors: Olga Zhaxybayeva and J Peter Gogarten

Cloning and characterization of the mouse Mcoln1 gene reveals an alternatively spliced transcript not seen in humans

Mucolipidosis type IV (MLIV) is an autosomal recessive lysosomal storage disorder characterized by severe neurologic and ophthalmologic abnormalities. Recently the MLIV gene, MCOLN1, has been identified as a new ...

Authors: John L Falardeau, John C Kennedy, James S Acierno Jr, Mei Sun, Stefanie Stahl, Ehud Goldin and Susan A Slaugenhaupt

Molecular diversity of phospholipase D in angiosperms

The phospholipase D (PLD) family has been identified in plants by recent molecular studies, fostered by the emerging importance of plant PLDs in stress physiology and signal transduction. However, the presence...

Authors: Marek Eliáš, Martin Potocký, Fatima Cvrčková and Viktor Žárský

Characterization of BTBD1 and BTBD2, two similar BTB-domain-containing Kelch-like proteins that interact with Topoisomerase I

Two-hybrid screening for proteins that interact with the core domain of human topoisomerase I identified two novel proteins, BTBD1 and BTBD2, which share 80% amino acid identities.

Authors: Lixin Xu, Lihong Yang, Keiko Hashimoto, Melvin Anderson, Glenda Kohlhagen, Yves Pommier and Peter D'Arpa

Gene conversion homogenizes the CMT1A paralogous repeats

Non-allelic homologous recombination between paralogous repeats is increasingly being recognized as a major mechanism causing both pathogenic microdeletions and duplications, and structural polymorphism in the...

Authors: Matthew E Hurles

Genomic organisation and alternative splicing of mouse and human thioredoxin reductase 1 genes

Thioredoxin reductase (TR) is a redox active protein involved in many cellular processes as part of the thioredoxin system. Presently there are three recognised forms of mammalian thioredoxin reductase designa...

Authors: Simone A Osborne and Kathryn F Tonissen

Identification of four families of yCCR4- and Mg²⁺-dependent endonuclease-related proteins in higher eukaryotes, and characterization of orthologs of yCCR4 with a conserved leucine-rich repeat essential for hCAF1/hPOP2 binding

The yeast yCCR4 factor belongs to the CCR4-NOT transcriptional regulatory complex, in which it interacts, through its leucine-rich repeat (LRR) motif with yPOP2. Recently, yCCR4 was shown to be a component of ...

Authors: Anne Dupressoir, Anne-Pierre Morel, Willy Barbot, Marie-Paule Loireau, Laura Corbo and Thierry Heidmann

Cloning, expression and nuclear localization of human NPM3, a member of the nucleophosmin/nucleoplasmin family of nuclear chaperones

Studies suggest that the related proteins nucleoplasmin and nucleophosmin (also called B23, NO38 or numatrin) are nuclear chaperones that mediate the assembly of nucleosomes and ribosomes, respectively, and th...

Authors: Gregory M Shackleford, Amit Ganguly and Craig A MacArthur

Genomic organization of Tropomodulins 2 and 4 and unusual intergenic and intraexonic splicing of YL-1 and Tropomodulin 4

The tropomodulins (TMODs) are a family of proteins that cap the pointed ends of actin filaments. Four TMODs have been identified in humans, with orthologs in mice. Mutations in actin or actin-binding proteins ...

Authors: Patrick R Cox, Teepu Siddique and Huda Y Zoghbi

Characterization of the mouse Dazap1 gene encoding an RNA-binding protein that interacts with infertility factors DAZ and DAZL

DAZAP1 (DAZ Associated Protein 1) was originally identified by a yeast two-hybrid system through its interaction with a putative male infertility factor, DAZ (Deleted in Azoospermia). In vitro, DAZAP1 interacts w...

Authors: Tiane Dai, Yanira Vera, Eduardo C Salido and Pauline H Yen

Erratum to: Genomic organization and alternative splicing of the human and mouse RPTPρ genes: Correction

Authors: Julie A Besco, Adrienne Frostholm, Magdalena C Popesco, Arthur HM Burghes and Andrej Rotter

High-throughput genotyping of single nucleotide polymorphisms with rolling circle amplification

Single nucleotide polymorphisms (SNPs) are the foundation of powerful complex trait and pharmacogenomic analyses. The availability of large SNP databases, however, has emphasized a need for inexpensive SNP gen...

Authors: A Fawad Faruqi, Seiyu Hosono, Mark D Driscoll, Frank B Dean, Osama Alsmadi, Rajanikanta Bandaru, Gyanendra Kumar, Brian Grimwade, Qiuling Zong, Zhenyu Sun, Yuefen Du, Stephen Kingsmore, Tim Knott and Roger S Lasken