Gene and transposable element methylation in great tit (Parus major) brain and blood

Background Studies on vertebrate DNA methylomes have revealed a regulatory role of tissue specific DNA methylation in relation to gene expression. However, it is not well known how tissue-specific methylation varies between different functional and structural components of genes and genomes. Using whole-genome bisulfite sequencing data we here describe both CpG and non-CpG methylation profiles of whole blood and brain tissue in relation to gene features, CpG-islands (CGIs), transposable elements (TE), and their functional roles in an ecological model species, the great tit (Parus major). Results We show that hypomethylation at the transcription start site (TSS) is enriched in genes with functional classes that relate directly to processes specific to each tissue type. We find that 6877 (~21 %) of the CGIs are differentially methylated between blood and brain, of which 1186 and 2055 are annotated to promoter and intragenic regions, respectively. We observe that CGI methylation in promoter regions is more conserved between tissues compared to CGI methylation in intra and inter-genic regions. Differentially methylated CGIs in promoter and intragenic regions are overrepresented in genomic loci linked to development, suggesting a distinct role for CGI methylation in regulating expression during development. Additionally, we find significant non-CpG methylation in brain but not in blood with a strong preference for methylation at CpA dinucleotide sites. Finally, CpG hypermethylation of TEs is significantly stronger in brain compared to blood, but does not correlate with TE activity. Surprisingly, TEs showed significant hypomethylation in non-CpG contexts which was negatively correlated with TE expression. Conclusion The discovery that TSS methylation levels are directly linked to functional classes related to each tissue provides new insights in the regulatory role of DNA-methylation patterns. The dominant sequence motifs for brain non-CpG methylation, similar to those found in mammals, suggests that a conserved non-CpG regulatory mechanism was already present in the amniote ancestor. The negative correlation between brain non-CpG methylation and TE activity (not found for CpG methylation) suggests that non-CpG is the dominant regulatory form of methylation in TE silencing. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-2653-y) contains supplementary material, which is available to authorized users.

Background DNA methylation is the addition of a methyl (-CH3) group to the 5' carbon site of cytosines catalyzed by DNA-methyltransferases, which occurs mainly at CpG sites in animals [1]. It is involved in many biological processes including modulation of gene expression. The majority of methylation studies have been conducted on either humans or model species for human diseases, and studies on ecological model systems are rare. Moreover, studies on such systems, where reference genomes are often lacking, mainly use targeted methods such as MS-AFLP or RRBS that rely heavily on assumptions from human and rodent studies and provide only limited functional insights [2]. We assessed whole genome DNA methylation in the great tit, a model organism for ecological and evolutionary studies on e.g. the effects of climate change on natural populations [3], the allocation of resources to breeding [4], the impact of variation in personality traits on other life history characters [5] and one of the first in the class of Aves to study the methylome [6]. Whole methylome information will give us detailed insight into processes related to gene expression, silencing and tissue specialization, and allows us to better predict what variation is important for answering ecologically relevant questions.
Previous studies on DNA methylation in animals have focused mainly on CGIs, which are often found near the TSS and are usually hypomethylated [7]. Methylation in promoter regions is often found to be negativelycorrelated with gene expression, becoming less accessible for transcription factors or RNA-polymerases [8]. However, not all functions and mechanisms of DNA methylation are well understood. For instance, a positive correlation is observed between gene expression and gene body (GB) methylation in many mammalian tissues [9]. In contrary, a slightly negative correlation was found in brain neural tissue [6,10]. Previous studies have demonstrated that GB methylation may have a role in splicing [11,12]. However, recent evidence contradicts this [13]. Other studies show that it could prevent interruptive transposable element (TE) insertions [14] and DNA methylation is known to suppress the activity of TEs in both plants and animals [15]. However, the underlying mechanisms of TE silencing differ between plants and animals [16]. In plants this involves small RNA-guided DNA methylation of cytosines resulting in a specific signature of dense methylation in all sequence contexts (CpG and non-CpG) [17], whereas a similar system in mammals is unknown, and DNA methylation in TEs and genes is almost exclusively found in a CpG context.
The methylation landscape can vary between tissue types, playing a large role in for example gene regulation [18]. Previous studies have identified differentially methylated regions across tissues [19][20][21]. However, these studies were mainly conducted in mammals and did not focus specifically on gene features, but rather on differentially methylated regions throughout the genome. The observation that DNA methylation negatively correlates with gene expression suggests that hypomethylation occurs in gene promoter regions that are associated with the biological functions of the tissue. Interestingly, in mammals CGI methylation levels were found to be more similar in promoter regions between tissue types compared to intra and intergenic CGIs [20]. Furthermore, CGIs showing differences in methylation patterns between tissues were overrepresented at loci essential for development (ectoderm and mesoderm development, neurogenesis and segment specification) in human and mouse [20,21], suggesting a distinct role for CGI methylation in development.
In addition to CpG-methylation, CHG and CHH trinucleotide sites (where H represents any nucleotide but G) can be methylated as well, and this type of methylation is reported for somatic tissues including brain, muscle and placenta [6,[22][23][24]. Non-CpG methylation accumulates during early postnatal development in the mammalian brain and seems to be driven specifically by the DNA methyltransferase DNMT3A [25]. CpA dinucleotide sites are predominantly methylated compared to CpT and CpC sites in mammals, and are starting to be linked to gene regulation in the brain [25,26]. In addition, the methyl CpG binding protein 2 (MeCP2) binds to methylated non-CpG sites, with a high affinity for mCpA dinucleotide sites. The function of non-CpG methylation and the binding of MeCP2 in brain tissue remains largely unclear, but some studies hypothesize a role for MeCP2 as a transcriptional repressor [27], whereas others propose a role as a transcriptional activator [28].
To expand our understanding of DNA methylation and its possible functions in birds, we investigated both CpG and non-CpG whole genome DNA methylation in brain and blood of a passerine bird (great tit, Parus major). We specifically assess methylation patterns and their functional roles in relation to gene features, CGIs, and TEs.

The great tit methylome
We performed whole genome bisulfite sequencing in brain and blood samples from a single adult male great tit recently used for genome assembly and annotation [6]. A total of 12.2 (blood) and 10.6 (brain) million CpG sites with a minimum depth of 10x were covered, representing 80 and 69 % of the total CpG sites in the genome, respectively. We observe a higher average CpG methylation level (the ratio of methylated reads to all reads covering a specific site) in brain compared to blood, based on 10,246,241 CpG sites covered (>10x) in both tissues (50.0 and 42.7 %, respectively; Table 1). The large majority of covered CpG-sites were methylated (relative methylation >10 %) in both brain (73.0 %) and blood (70.2 %). These numbers are consistent with previous findings in mammals [29,30]. We observe significant non-CpG methylation in brain (CHG: 3.4 %, CHH: 5.5 %) but not in blood (Table 1). For blood, 97 % of methylated Cs were derived from CpG sites (Fig. 1). In brain, the majority of methylated Cs (52 %) were located at non-CpG sites, but with generally lower methylation levels. The higher general methylation level in brain is due to a larger proportion of fully methylated CpG-sites (>80 %) (Fig. 2). Similar analysis for non-CpG methylation in brain shows that both CHG and CHH sites have generally no or very low methylation levels (<20 %) and similar distributions (Additional file 1: Figure S1). The majority of non-CpG methylation in the great tit brain occurs at CpA dinucleotide sites (75 %), representing 72 and 88 % of methylated CHH and CHG sites, respectively ( Table 2, Additional file 1: Figure S2). Additional non-CpG methylation mainly occurs at CpT sites (22 %), with CpC dinucleotide sites being rarely methylated (3 %). Furthermore, the average methylation level for CpA sites is 3.4 %, 1.16 % for CpT sites, and only 0.31 % for CpC sites (Table 2).
Further sequence analysis revealed a dominant CAC sequence motif for CHH methylated sites (Fig. 3). These motifs are consistent with previous findings in mammals [26,31], but have not been described previously in Aves.
In order to assess the methylation levels in genic regions we divided the genes into specific gene features: TSS (300 bp upstream -50 bp downstream of the annotated TSS), five prime untranslated region (5'UTR), GB, coding sequence (CDS), introns, three prime untranslated regions (3'UTR) and transcription termination site (TTS; 50 bp upstream -200 bp downstream of the annotated TTS). Methylation levels were further calculated in the context of gene regions by dividing each gene into overlapping sliding windows and including the 10 kb upstream and downstream regions (Additional file 1: Figures S3, S4). We found low methylation levels in TSS and 5'UTR regions and higher methylation levels in the GB increasing towards the 3'UTR before decreasing again near the TTS. We found higher methylation levels in coding regions compared to intronic regions (Additional file 1: Figure  S5) and longer genes have generally higher CpG methylation levels (Fig. 4).  1 Relative proportion of methylated Cs (methylation level >10 %) in brain and blood for three sequence contexts. The majority of methylated Cs were located at CpG sites in blood, compared to CHH and CHG sites in brain Differential CpG methylation between blood and brain We identified genes differentially methylated between brain and blood in three gene features; TSS, GB, and TTS. Based on the fold change calculated from the relative methylation in the two tissue types, we extracted the 5 % of genes with most extreme methylation fold changes (upper and lower 2.5 %) between tissues for enrichment analysis (Table 3). For TSS, GB, and TTS these sets included 382, 410, and 289 genes, respectively. We found very little overlap in the set of differentially methylated genes from the three gene features (Additional file 1: Figure S6). Gene ontology (GO) enrichment analysis showed that genes with a hypomethylated TSS in the brain compared to blood were enriched in categories linked to regulation of axonogenesis, neuronal synaptic plasticity, neurogenesis, and spindle localization (Additional file 2: Table S1). In contrast, genes with a hypomethylated TSS in blood compared to brain were largely enriched in categories linked to lymphocyte and leukocyte cell activation, immune response, and hemopoiesis (Additional file 2: Table S2). Many of these enriched biological processes correspond directly to the specific functions of the examined tissues. Moreover, this result suggests that, at least in brain and blood, genes are generally TSS-hypomethylated in the tissue they are expected to be expressed in. Genes with hypermethylated GBs in brain compared to blood are involved in the (positive) regulation of transcription, development (cell fate commitment, regionalization, embryonic and organ development/morphogenesis), and regulation of immune system process (Additional file 2: Table S3). GB hypomethylated genes in brain compared to blood are involved in transcription, RNA-splicing, and translation (Additional file 2: Table S4). These results suggests that genes involved in transcription, splicing, and translation are generally GB-hypomethylated in brain to enhance their transcription, while genes important for developmental processes are GB-hypermethylated to silence their transcription, supported by significant differences in brain gene expression data between the two sets (TSS; p < 1x10 −10 , GB; p < 1x10 −9 , TTS; p < 0.01; Table 3). Genes with hypermethylated TTS in brain compared to blood are involved in the negative regulation of transcription, opposite of GBhypermethylated genes (Additional file 2: Table S5). The difference in expression in relation to brain methylation supports the observed negative correlation between Fig. 2 Distribution of methylation level in blood and brain for mCpG-sites. Methylated CpG sites (methylation level > 0 %) covered in both tissues are divided into ten bins according to their methylation level on the x-axis. The y-axis indicates the proportion of total CpGs that are methylated within each bin. A higher abundance of hypermethylated (methylation level > 80 %) CpG sites was observed in brain compared to blood  Figure S7).

CGI CpG methylation
We divided the CGIs into three groups according to the position of the CGI: promoter associated CGIs (2 kb upstream -500 bp downstream of annotated TSS), intragenic CGIs, and intergenic CGIs. While promoter CGIs in general tend to be hypomethylated, intragenic CGIs tend to have higher methylation in both tissues (Additional file 1: Figure S8). Moreover, when comparing the methylation levels of these CGIs in blood and brain, we found fewer differentially methylated CGIs in promoter regions (11.9 %) compared to intragenic and intergenic CGIs (23.0 and 25.3 %, respectively; Table 4). We found that genes associated with hypermethylated CGIs (both promoter and intragenic) in brain compared to blood are largely involved in developmental processes (morphogenesis, cell differentiation, organ development, embryonic development, and system development; Additional file 3: Tables S1, S2). These results are consistent with previous studies where differential methylation across tissues was found in genes linked to development [20,21]. Genes associated with hypomethylated promoter CGIs in brain compared to blood are involved in the protein secretion of platelet, neurotrophin production, and regulation of protein targeting to membrane (Additional file 3: Table S3), while genes with hypomethylated intragenic CGIs in brain are enriched for categories linked to (neuron) development and differentiation (cell differentiation, system development, generation of neurons and neurogenesis; Additional file 3: Table  S4). We observe significantly higher brain expression of genes associated with both promoter and intragenic hypomethylated CGIs compared to hypermethylated CGIs in brain (Promoter: p < 1x10 −05 , Intra: p < 1x10 −07 ; Additional file 1: Table S1).

Non-CpG gene methylation in brain tissue
To discover if there is a functional signal related to non-CpG methylation we divided all genes according to their non-CpG methylation level in brain and used the upper and lower 2.5 % to perform GO enrichment analysis. Genes with no or very low non-CpG methylation (lower 2.5 %) are largely involved in transcriptional/translational processes (gene expression, mRNA metabolic process, ribosome biogenesis, and translation; Additional file 4: Tables S1-S3). The genes with the highest methylation (upper 2.5 %) in terms of TSS and TTS non-CpG methylation are mainly involved in system development, glial cell differentiation, gliogenesis and glial cell fate specification (Additional file 4: Tables S4, S5), while genes with highly non-CpG methylated GB are frequently involved in processes linked to the immune system (adaptive immune response, leukocyte mediated immunity, B cell mediated immunity) and cell adhesion (Additional file 4: Table S6). Genes in the lower 2.5 % have significantly higher expression (TSS; p < 0.001, GB; p < 1x10 −11 , TTS; p < 1x10 −06 ) compared to the highly non-CpG methylated genes (Additional file 1: Table S2). In addition, we found a positive correlation between non-CpG methylation level and gene length (Additional file 1: Figures S9, S10), a pattern consistent with recent reports in mammals [32,33]. This suggests that the observed differences are biased towards longer genes, where genes involved in protein, DNA, and RNA metabolism are generally shorter compared to genes in the upper non-CpG methylation percentiles (involved in gliogenesis, cell-adhesion or immune response). Additionally, a negative correlation between non-CpG methylation levels and gene expression was observed in all gene features, earlier described by Laine et al. 2016 [6] (Additional file 1: Figure S11).

Transposable element methylation
We assessed DNA methylation in 26,834 TEs (mainly LINE/CR1 and LTR/ERVL elements) divided into short interspersed elements (SINE), long interspersed elements (LINE), and LTR retrotransposons. CpG sites within TEs are largely hypermethylated in both brain and blood (Additional file 1: Figure S12). The CpG methylation levels in TEs and upstream and downstream 2 kb flanking regions in brain are significantly higher than in blood, with the difference in average methylation reaching up to 20 %. Interestingly, the opposite pattern was observed for non-CpG methylation, with decreased non-CpG methylation in TEs compared to their flanking regions in brain (Additional file 1: Figure S13). Additionally, TE expression shows a negative correlation with non-CpG methylation level (Spearman's rho < −0.11, P < 1x10 −15 ; Fig. 5). This negative correlation is even stronger in the 2 kb upstream (Spearman's rho < −0.20, P < 1x10 −15 ) and downstream regions (Spearman's rho < −0.19, P < 1x10 −15 ). Surprisingly this negative correlation was not observed for CpG methylation (Spearman's rho: TE-body = 0.033, upstream = −0.0056, downstream = −0.0023; Additional file 1: Figure  S14). The methylation patterns did not differ between the different types of TEs.

Independent validation of RNA-seq and WGBS results
In order to validate the RNA-seq gene expression results, we performed qPCR on 11 randomly chosen genes using RNA isolated from whole brain tissue of the same individual, as well as five additional individuals. The results revealed a highly significant positive correlation between the RNA-seq and qPCR gene expression results (Pearson correlation; r = 0.753, p < 0.001)(Additional file 1: Figure S15). In order to validate the WGBS methylation results, we We used the upper and lower 2.5 % of genes based on fold-change to identify differentially methylated genes with the sum of both average methylation levels > 10 % in order to exclude hypomethylated genes in both tissue types. The fourth (brain hypomethylated) and fifth (brain hypermethylated) column show average and median brain gene expression values expressed as fragments per kilobase of transcript per million mapped fragments (FPKM) for the set of genes with differentially methylated gene features We calculated the relative methylation levels for CGIs with at least three CpG sites, and determined differentially methylated CGIs using Fisher's exact test. Table shows the number of differentially methylated CGIs and associated genes in blood and brain generated reduced representation bisulfite sequencing (RRBS) data using DNA isolated from blood samples from two additional individuals. An average non-CpG methylation level of <0.4 % was observed, with only~2 % of the methylated sites (relative methylation >10 %) derive from non-CpG sites in the two RRBS samples (Additional file 1: Table S4), confirming the lack of non-CG methylation in the blood. Furthermore, we used the two RRBS samples to perform correlation analysis on 2000 randomly selected CpG sites (coverage > 10x in all samples) ranging in methylation level between 0 and 100 % in the WGBS sample. We observed a high correlation between the WGBS and both RRBS samples, with Pearson correlation coefficients of 0.758 (p < 0.001) and 0.767 (p < 0.001), respectively (Additional file 1: Figure S16). Together, these results validate the RNA-seq and WGBS results presented here.

Discussion
Until recently, chickens were the only birds with DNA methylation information available [34]. The recent publication of the great tit genome has revealed methylation patterns analogous to those of mammals [6]. However, the observed dominant sequence motifs for non-CpG methylation have not been previously described in the Aves class. We have shown differential methylation levels between brain and blood for groups of genes linked to the functions of those tissues. Furthermore, differentially methylated CGIs between brain and blood that are located in promoter and intragenic regions are overrepresented in genetic loci essential for development. TEs are hypermethylated at CpG sites but hypomethylated at non-CpG sites. Interestingly, TE expression in brain tissue negatively correlates with non-CpG methylation, but not with CpG methylation. This novel finding provides new insights in the mechanism of TE silencing by methylation in the great tit brain. We observed a positive correlation between gene length and methylation level (both CpG and non-CpG). One explanation is that larger genes can compensate for lowly methylated TSS and 5'UTR regions, since they consist of a relatively larger GB. This, however, does not explain that genes in the first size rank (smallest genes) show higher methylation in CpG context. Since single exon genes will be more predominant in the lower rank, with the methylation levels being higher in codingsequences compared to intronic regions, this might explain the unusually high methylation levels of the smallest gene set.
There is a very strong association between TSS hypomethylation and gene function; genes with brain related functions were also hypomethylated in brain TSS, supporting that decreased TSS methylation is involved in very specific gene regulation. Interestingly, this signal was not observed for promoter CGIs. In general, methylation levels are conserved in promoter CGIs between the two tissues, being hypomethylated in both brain and blood, supporting the generalization that CGI methylation silences promoters. Intragenic CGIs tend to show higher methylation levels. Hypermethylated promoter and intragenic CGIs in brain are associated with genes involved in development. One hypothesis is that CGI hypermethylation in genomic loci linked to development could silence transcription after brain development. Following human germ cell development, the genome is demethylated and remethylated [35]. The opposing hypermethylation of intragenic CGIs in adult brain and hypomethylation in blood could further be explained by the negative correlation between gene body methylation and expression in brain [10] and the positive correlation in blood [36]. However this does not explain the difference between adult blood and brain methylation levels at promoter CGIs.
Non-CpG methylation in (passerine) birds seems to be restricted to certain tissue types and we only found this in brain. No or very low non-CpG methylation was found for genes involved in transcriptional and translational processes. In addition, these genes show higher expression compared to the average. We therefore suggest that highly expressed genes have very little or no non-CpG methylation to enhance transcription. We found a dominant CAG and CAC sequence motif for both CHG and CHH methylated sites, as previously described in mammals [26,31]. This supports the existence of a shared non-CpG regulatory mechanism between birds and mammals. We could even argue that non-CpG methylation occurs predominantly on CWG and CWH sites (W = A or T), because CC dinucleotide sites are hardly methylated. The observed dominant motifs are associated with a stronger binding affinity of MeCP2 (especially the methylated CpA dinucleotide sites) in mammals [32]. MeCP2 has also been shown to preferentially silence longer genes enriched with methylated CpA sites [32]. This could explain why we find no or very low CpA methylation in genes involved in protein, DNA, and RNA metabolism (which are generally shorter genes), but do find it in longer genes (e.g. genes involved in cell adhesion). However, the exact function of MeCP2 binding to non-CpG methylated sites remains largely unclear and is associated with both transcriptional silencing and activation [27,28].
The large differences we found in general methylation levels between brain and blood can be explained by a larger proportion of fully methylated CpG sites in brain, where in blood moderate methylation is more abundant. This dissimilarity might in part be attributed to hypermethylated TEs in brain, which are methylated at lower levels in blood. Methylation of TEs decreases or inhibits transpositional activity. Previous studies observed large differences in TE methylation levels between tissues [37], although not specifically for blood and brain. Opposite to CpG methylation, we found decreased non-CpG methylation in TEs compared to their flanking regions. In addition, our observation that non-CpG methylation negatively correlates with TE activity suggests a distinct role for non-CpG methylation in TE silencing in the brain. Surprisingly, we did not observe any correlation between CpG methylation and TE activity. Recent studies have shown that TEs are more active in brain tissue compared to other tissues in mammals [38]. Based on our results, we hypothesize that non-CpG methylation is involved in, and is perhaps even the main regulator of TE silencing in brain.
It is important to note that although we observe a role for brain methylation in regulating gene expression and TE activity, the brain consists of several highly distinctive regions with a variety of functions. Therefore, the results presented here must be taken as the average for whole brain, and cannot be directly correlated with individual brain regions. Moreover, since we only used two tissue types in our analysis, we can only draw conclusions about differences among these tissues. To make more general conclusions about tissue-specificity, more tissues need to be included in the future. Nevertheless, these results provide new insights and present new possibilities to further exploration of the role of methylation (especially the role of non-CpG methylation in TEs) in specific brain regions. In addition, while whole genome bisulfite sequencing is considered the gold standard technique for analyzing genome-wide DNA methylation patterns, the high cost associated with producing such datasets limits the number of biological replicates in studies such as this one. Therefore, although performing statistical analysis at the genic level allows for robust statistical analysis within a single sample, and no non-CpG methylation was detected in the whole blood sample (as expected), further studies in additional individuals are required to confirm the results presented here.

Conclusion
We show distinct methylation patterns of two tissue types in the great tit. In addition, we observe a clear link between methylation and the regulation of gene expression and TE activity in the brain. The dominant CAG and CAC sequence motifs for brain non-CpG methylation, which are also observed in mammals, suggests a conserved non-CpG regulatory mechanism is present between birds and mammals. From our results, we hypothesize a distinct function for CpG and non-CpG methylation in TE silencing in the brain. This theory is supported by the contrast between CpG and non-CpG methylation in TEs. Moreover, a negative correlation between non-CpG methylation and TE activity (not found for CpG methylation) shows that non-CpG methylation is involved in TE silencing and perhaps the dominant regulatory form of methylation in the brain.

Sample collection
Whole brain was collected from a single adult male bird, snap-frozen, and stored in RNAlater (Thermo Fisher Scientific) until processing. This bird is the same as the reference genome individual [6]. Whole blood was collected from the same individual from the carotid artery after euthanization. Approval was received for this from the Animal Experiment Committee from the Royal Netherlands Academy of Sciences (DEC-KNAW) under protocol number CTE-0705 Adendum I.
Whole genome bisulfite sequencing library preparation and sequencing DNA was extracted from whole blood using a Gentra Puregene Kit (Qiagen, USA) following the manufacturer's protocol. Homogenized whole brain was incubated overnight at 55°C in 750 μl Cell Lysis Solution (Gentra Puregene Kit, Qiagen, USA) with 20 μl proteinase K. 250 μl of this lysed tissue was added to 250 ul Cell Lysis Solution. To remove excess of fat and proteins, 500 μl 24:1 chloroform:isoamylalcohol was added and mixed until homogeneous, followed by 10 min centrifugation at 12.000x g after which the upper layer was collected. Cell Lysis Solution was added to this upper layer until 500 μl of sample liquid was obtained and total DNA was extracted according to the manufacturer's protocol. DNA was stored in DNA Hydration Solution (Qiagen, USA), and the concentration was determined with a Nanodrop 2000 (ThermoThermo Scientific, USA). DNA was sheared using a Covaris E210 device tõ 700 bp peak fragment sizes. One microgram sheared and purified DNA including 1 ng sheared Lambda DNA was used for end repair, adenylating and adapter ligation according to illumina TruSeq LT DNA sample preparation guide. Adapter Ligated DNA was purified using AmpureXP beads (Agencourt) and subsequently used for bisulfite conversion according to EpiTect Plus Bisulfite workflow (Qiagen) with small modifications i.e. three additional rounds of denaturation's (5 min at 95°C each) and incubations (60°C for 25, 85 and 125 min respectively). Converted DNA was purified following manufacturer's instructions (EpiTect) and split over three parallel reactions for library amplification using Pfu Cx hotstart DNA polymerase (Agilent Technologies) and 18 PCR cycles. PCR products were pooled per sample and final amplified bisulfite converted libraries were quantified using Qubit (Invitrogen) and Bio analyzer DNA 1000 chip (Agilent Technologies). Libraries were used for clustering on two partial flowcells on an Illumina HiSeq 2000 system generating 358.3 M (72.4Gbp) and 292.4 M (59.1Gbp) paired-end reads (101 bp) for brain and blood, respectively.

Whole genome bisulfite sequencing analysis
Raw reads were trimmed for quality (>20) and adapter sequences using trim_galore v.0.1.4 (http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/), producing a cleaned set of 334.8 M and 274.8 M paired end reads for brain and blood, respectively. These reads were aligned to the great tit reference genome [6] using BS-Seeker v2.0.6 [39] with Bowtie2 v2.1.0 using the local alignment mode [40]. In total, 95,79 and 98.04 % of the genome was covered with an average depth of 31.89x (52 % mapping rate) and 33.04x (64 % mapping rate) in brain and blood, respectively. Methylation levels (defined as the ratio of methylated/total reads at a given site) were determined using the methylation call scripts from BSseeker2. All downstream analysis was performed using sites covered by a minimum of 10 reads.

Methylation variation across gene features
Each gene is subdivided into TSS (300 bp upstream, 50 bp downstream of annotated TSS), GB (representing the full length of the annotated gene), and TTS (50 bp upstream, 200 bp downstream of annotated TTS). Transcripts with similar TSS and TTS boundaries were merged to use a set of unique transcripts for downstream analysis. We calculated the fold-change difference in average CpG methylation level between blood and brain in all three gene features (TSS, GB, TTS) where the sum of the relative methylation level in blood and brain is at least 10 % (to exclude hypomethylated gene features in both tissues) and with at least three CpG sites covered. We used the upper (hypermethylated) and lower (hypomethylated) 2.5 % of genes to define differentially methylated regions. We calculated the average non-CpG methylation in blood and brain for TSS, GB, and TTS with at least three non-CpG sites covered. We divided each region into 40 groups of percentiles based on their non-CpG methylation levels using a custom python script and the upper (40th) and lower (1st) percentiles were defined as differentially methylated. The GB was further subdivided into 4 different GB features using custom python scripts; 5'UTR, CDS, intronic sequences, and 3'UTR. We calculated the average methylation levels for both CpG and non-CpG sites covering these GB features. In addition, we used a sliding window approach to correlate the methylation level with gene expression in brain (Additional file 1: Figures S7-S11). We used three different gene regions; the GB, 10 kb upstream, and 10 kb downstream of the gene boundaries, For each region, we used 40 overlapping sliding windows (5 % of the regions length) with an overlap of 2.50 %. We subdivided the genes in 10 percentiles based on gene length to assess the correlation between gene length and relative methylation level (over the full length of the annotated gene).

Non-CpG methylation sequence motifs
Custom python scripts were used to retrieve sequence motifs (3 bp upstream and 5 bp downstream of the methylated site) and WebLogo3.3 was used to build sequence logos [41].

CGI prediction and differential methylation
CGIs were predicted using the cpgplot software, part of the EMBOSS package (version 6.6.0.0) applying default settings (−window 100 -minlen 200 -minoe 0.6 -minpc 50). In total 33,131 CGIs were identified in the genome. CGIs were associated with a promoter if the CGI was annotated within 2 kb upstream to 500 bp downstream of a gene's annotated TSS. A CGI was considered intragenic if annotated within the boundaries of a gene, or if it completely covered the gene. We calculated the relative methylation levels for CGIs with at least three CpG sites, and differentially methylated CGIs were determined using a fisher exact test. Bonferroni correction was used to account for multiple testing, q-value < = 0.05 is considered significant.

RNA-seq library preparation and sequencing
RNA was extracted from homogenized whole brain using the miRNeasy mini kit (Qiagen) following the manufacturer's protocol. For library preparation we used the TruSeq Stranded RNA Sample Preparation Kit (Illumina) using a barcoded adapter. Sequencing was performed on an Illumina HiSeq 2000. This resulted in 229.6 M (46.4Gbp) paired-end 100 bp RNA-seq reads.

RNA-seq analysis
FastQC (http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) was used to check the quality of the sequences and low-quality bases were trimmed off using Fastq-mcf [42]. In total, 200.8 M trimmed paired end reads were aligned (84.7 % overall mapping rate) against the reference genome with Tophat v2.0.10 using -maxmultihits 20, -read-realign-edit-dist 0,-mate-inner-dist 50,-mate-std-dev 150 [43]. Tophat analysis included a pre-alignment to the reference genome to filter out reads extending the maximum number of alignments (-M option) followed by alignment to the reference transcriptome (-G) and alignment to the genome. Transcript assembly and quantification was done with Cufflinks v2.2.0 including the annotation (-g option),-overlap-radius 5 and -intron-overhang-tolerance 5. Expression values were extracted from the cufflinks output and represented as fragments per kilobase of exons per million mapped reads (FPKM) [43]. We used a two sample t-test assuming equal variances to test for significant gene expression differences between differentially methylated gene sets. Spearman's correlations between gene expression and methylation were calculated using R (version: 3.0.2).

Gene ontology (GO) analysis
We performed blastp (e-value 0.1) [44] to human, mouse, and chicken, and InterProScan [45] on the total great tit gene set. Out of 16,424 transcripts, 15,186 were assigned to a total of 247,144 GO-terms (Additional file 1: Table S3).
These results were imported into Blast2GO to assign GOterms for each individual gene [46]. We calculated the foldchange difference in average CpG methylation level between blood and brain in all three gene features (TSS, GB, TTS) where the sum of the relative methylation level in blood and brain is at least 10 % (to exclude hypomethylated gene features in both tissues) and with at least three CpG sites covered. We used the upper (hypermethylated) and lower (hypomethylated) 2.5 % of gene features to define differentially methylated regions for gene ontology analysis. We calculated the average non-CpG methylation in blood and brain for TSS, GB, and TTS with at least three non-CpG sites covered. We divided the regions into 40 groups of percentiles based on their non-CpG methylation levels (minimum 3 non-CpG sites) using a custom python script and the upper (40th) and lower (1st) percentiles were defined as differentially methylated and used for GOenrichment analysis. The CytoScape plugin BINGO [47] was used for GO enrichment analysis using a hypergeometric test to calculate p-values and a Benjamin & Hochberg False Discovery Rate (FDR) correction to calculate qvalues [47]. The GO annotations from Blast2GO were used as the reference data set. GO-terms with a q-value less than or equal to 0.05 were considered enriched.

Transposable elements
Transposable elements (TE) were annotated using RepeatMasker (v4.0.3) [48] with RepBase (update: 20130422) [49]. In total, 26,834 TEs covering 2.1 % (21.3 Mb) of the genome were used in the analysis with TEs smaller than 500 bp excluded. We used a sliding window approach to assess the relative methylation levels of TEs by divided them into three different regions; the TE, 2 kb upstream and 2 kb downstream. Each region was subdivided into ten overlapping sliding windows (20 % of the regions length) with an overlap of 10 %, and average methylation levels were calculated for each sliding window. We used Tophat version 2.0.10 to uniquely align the RNA-seq reads (84.3 % overall mapping rate) to the reference genome [43] with following settings: max-multihits 1,-read-realign-edit-dist 0,mate-inner-dist 50 and -mate-std-dev 150. TE expression was assessed using the cufflinks -GTF option with a GFF containing all identified TEs [43] and -overlap-radius 5,-intron-overhang-tolerance 5. TE expression values (FPKM) were extracted from the Cufflinks output. Spearman's correlations between TE expression and methylation were calculated using R (version: 3.0.2).
Validation of RNA-seq via quantitative real-time PCR (qPCR) RNA was extracted from hypothalamus tissue of five great tits collected for another study (Verhagen unpublished.). Punches were taken from the hypothalamus region using the method of Perfito et al. 2012 [50]. In short, brain tissue was cut on a cryostat. 3 mm circular punches were sampled from the hypothalamus region, resulting in sampling equal volumes from each individual brain. Brain punches were immediately added to 1 ml TRIzol Reagent (Thermo Fisher Scientific, Waltham, USA), homogenized and stored at -80C until extraction. Total RNA of brain punches was extracted using the method of TRIzol Reagent. For these five individuals and the single reference individual used for the other analyses (for RNA extraction see RNA-seq library preparation and sequencing) 354 ng of RNA was used to synthesize first-strand cDNA with QuantiTect Reverse Transcription Kit (Qiagen, Venlo, The Netherlands) following the standard protocols. RNA concentration was quantified using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific) and an AATI Fragment Analyser (Advanced Analytical Technologies, Heidelberg, Germany). The expression level of 12 genes was further investigated by qPCR. The qPCR analysis was performed with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific) in a C1000 Touch CFX96 (Biorad, Veenendaal, the Netherlands) Primers used for qPCR analysis are given in Additional file 1: Table S5. Great tit GAPDH (augustus_masked-chr1-processed-gene-22.8) was used as a standard control. The qPCR program was performed as follows: 50°C (2 min), 95°C (2 min), (95°C (15 s), 60°C (1 min))x40 cycles. PCR fragment sizes for the 12 genes were visualized on 1 % agarose gel to verify their band size and specificity.

Validation of WGBS via RRBS
For this we used blood samplings of two great tits originating from the F2 inter-cross between lines selected for high and low levels of exploratory behaviour [51]. Blood samples of 10 ul were collected when the birds were 40 days old and stored in 1 ml Cell Lysis Solution (Gentra Puregene Kit, Qiagen, USA). Total DNA was prepared by using 250 μl of the stored blood samples with 750 μl Cell Lysis Solution (Gentra Puregene Kit, Qiagen, USA) incubated with proteinase K at 55C overnight, followed by DNA extraction following the manufacturer's protocol. DNA was stored in DNA Hydration Solution (Qiagen, USA). Integrity of the DNA as well as absence of RNA was verified by running and visually analyzing 1.5 ul of DNA on a 1 % agarose gel next to a DNA ladder. High-quality genomic DNA (1 μg) was sent to the Carver High-Throughput DNA Sequencing and Genotyping Unit (University of Illinois, Urbana, IL, USA) for generation of RRBS libraries following standard protocols. Briefly, DNA was restriction digested using the methyl-insensitive restriction enzyme Mspl, and the resulting fragments were size-selected (20-200 bp) using agarose gel electrophoresis. Size-selected DNA was bisulfite-treated with the EpiTech Bisulfite Kit (Qiagen, Valencia, CA, USA) and column-purified. The final libraries were quantified using Qubit (Life Technologies, Carlsbad, CA, USA) and the average size was determined on an Agilent bioanalyzer DNA7500 DNA chip (Agilent Technologies, Wilmington, DE, USA) and diluted to 10 nM. The 10 nM dilution was further quantitated by qPCR on an ABI 1900 to ensure high accuracy quantification for consistent pooling of barcoded libraries and maximization of the number of clusters in the Illumina flowcell. RRBS Illumina sequencing was performed on libraries multiplexed and loaded onto 8-lane flowcells for cluster formation and sequenced on an Illumina HiSeq2500. The libraries were sequenced to a total read length of 100 bp from one end (single-end sequencing) of the molecules. Raw reads were trimmed using trim_galore v 0.4.1 and aligned to the great tit reference genome [6] using BS-Seeker v2.0.10 in -rrbs mode [39] with Bowtie2 v2.2.7 using the local alignment mode [40]. This resulted in a mappability of 75.43 and 75.94 %. Methylation levels were determined using the methylation call scripts from BSseeker2 with a minimum depth of 10x. 2000 randomly selected CpG sites (coverage > 10x in all samples) ranging in methylation level between 0 and 100 % in the WGBS sample were extracted from the RRBS samples. Pearson correlations on the 2000 randomly selected CpG sites were calculated using R (version: 3.0.2).

Availability of data and materials
The raw methylome data has been deposited into the NCBI Short Read Archive (SRA) under BioProject PRJNA208335 in the study SRP055861 and are accessible via the following URLs: brain: http://www.ncbi.nlm.nih. gov/sra/?term=SRS964344 blood: http://www.ncbi.nlm. nih.gov/sra/?term=SRS964345. The raw RRBS data are accessible via the following URLs: http://www.ncbi.nlm. nih.gov/sra/?term=SRS1341021 and http://www.ncbi.nlm. nih.gov/sra/?term=SRS1340777. The raw brain RNA-seq data is accessible via the URL: http://www.ncbi.nlm.nih. gov/sra/?term=SRS866013. The version of the genome used in this paper can be found at GenBank under the accession GenBank:JRXK01000000. Datasets supporting the results of this article are also included in the additional files.

Additional files
Additional file 1: Figure S1. Methylation level distribution for non-CpG sites (>0 %). Figure S2. non-CpG dinucleotide methylation preferences. Figure S3. CpG methylation level distribution in genes. Figure S4. non-CpG methylation level distribution in genes. Figure S5. Average CpG methylation in different gene partitions. Figure S6. The overlap for brain differentially hypo-methylated (A) and hyper-methylated (B) gene features. Figure S7. CpG methylation in relation to gene expression in