Evaluation of whole exome sequencing as an alternative to BeadChip and whole genome sequencing in human population genetic analysis

Background Understanding the underlying genetic structure of human populations is of fundamental interest to both biological and social sciences. Advances in high-throughput genotyping technology have markedly improved our understanding of global patterns of human genetic variation. The most widely used methods for collecting variant information at the DNA-level include whole genome sequencing, which remains costly, and the more economical solution of array-based techniques, as these are capable of simultaneously genotyping a pre-selected set of variable DNA sites in the human genome. The largest publicly accessible set of human genomic sequence data available today originates from exome sequencing that comprises around 1.2% of the whole genome (approximately 30 million base pairs). Results To unbiasedly compare the effect of SNP selection strategies in population genetic analysis we subsampled the variants of the same highly curated 1 K Genome dataset to mimic genome, exome sequencing and array data in order to eliminate the effect of different chemistry and error profiles of these different approaches. Next we compared the application of the exome dataset to the array-based dataset and to the gold standard whole genome dataset using the same population genetic analysis methods. Conclusions Our results draw attention to some of the inherent problems that arise from using pre-selected SNP sets for population genetic analysis. Additionally, we demonstrate that exome sequencing provides a better alternative to the array-based methods for population genetic analysis. In this study, we propose a strategy for unbiased variant collection from exome data and offer a bioinformatics protocol for proper data processing. Electronic supplementary material The online version of this article (10.1186/s12864-018-5168-x) contains supplementary material, which is available to authorized users.


Background
The investigation of the ethnogenesis of human populations is made possible by population genetic studies, through comparing genetic makeup and frequencies of the selected variants or alleles, and also by computing their genetic distance from the rest of the studied population or their level of admixture [1,2]. Compared to their more costly whole genome sequencing (WGS) counterparts, these assays predominantly use array-based genotyping techniques (various Human BeadChip arrays /610, 640, 650, 1 M/, Infinium Multi-Ethnic BeadChip arrays from Illumina, Affymetrix Genome-Wide Human SNP Array, Affymetrix Human Origin Array, etc.) that include single nucleotide polymorphism (SNP) sets based on the evaluation of previous genome sequencing data (CEPH, HGP, HapMap databases), with the emphasis on population-specific, ancestry-informative markers (AIMs). AIMs were first introduced in 2008 by Halder and colleagues [3], as a panel of 176 autosomal AIMs that were capable of effectively distinguishing individual biogeographical ancestry and admixture proportions from among four continental ancestral populations. The importance of AIMs has palpable significance in the medical field as well. While case-control design studies can be an efficacious strategy for identifying candidate genes in complex diseases in a population, in diversely admixed populations (e.g. Latin Americans, with admixture of American Indians, Europeans and Africans) population stratification can affect association studies and thereby could lead to false genetic associations [4]. This undesirable distortion can be minimized by genotyping AIMs.
Ultimately, application of the Whole Exome Sequencing (WES) method had spread and gained popularity, as WES is cost effective for routine genetic diagnosis of rare inherited diseases, and extensive databases have been generated containing thousands of publicly accessible exomes (Exome Aggregation Consortium~61000 exomes [5], Exome Variant Server~6500 exomes [6]). A typical WES dataset contains 100,000 to 130,000 variants (if all the high coverage reads are also analyzed, including the flanking regions of exons). In rare disease diagnostics, focus is on filtering out all non-disease specific variants and to find the one or two causative mutations that lead to the disease phenotype. In practice, all rare and common variants aid the exploration of disease variants only as population controls. Since exome data by definition contains high portion of the functional variants that are under selection pressure, in this study, we explored whether this could lead to any bias in population genetic analysis.
Thus, we assessed the usability of exome data in comparison with the other approaches in population genetic analyses. In order to compare the practicality of each strategy -(the genome data as unbiased standard, the commonly used array data, and the potentially usable exome data)we generated three subsets of the same publicly available experimental data (HGP~2,500 unrelated genomes), accordingly (GENOME, EXOME and BEADCHIP datasets). The results we obtained from these datasets were compared using the most widely used population genetic calculations (including Fixation index (F ST ), Principal Components Analysis (PCA), f 3− and f 4− statistics, admixture) by utilizing the commonly used tools: EIGENSOFT [7], ADMIXTOOLS [8], Admixture [9] and TreeMix [10].
Migration, admixture, adaptation, and genetic drift lead to genetic diversity between human populations. By studying genetic diversity within and between populations, we could reconstruct how these populations are related to one another. Fixation index a measure of genetic structure developed by Sewall Wright [11,12] can be estimated from genetic polymorphism data. F ST is the proportion of the total genetic variance contained in a subpopulation (S) relative to the total genetic variance (T). Its values can range from 0 to 1, where F ST = 0 implies panmixia (absence of any differentiation among subpopulations) and F ST = 1 implies complete divergence between populations.
Principal Components Analysis is the most widely used approach for identifying ancestry differences among a group of individuals [13,14]. When applied to genotype data, it calculates principal components (or eigenvectors), which can be viewed as continuous axes of variation that reflect genetic variation due to ancestry in the sample. Individuals with similar values for a particular top principal component will have similar ancestry for that axes. Application of principal components to genetic data from European samples [13] showed that among Europeans for whom all four grandparents originated in the same country, the first two principal components computed using 200,000 SNPs could geographically map their country of origin quite accurately.
F-statistics measure shared genetic drift among sets of populations and can be used to test simple and complex hypotheses about admixture events between populations [15]. The f 3 -statistics is used for testing the relationship of three populations [16] by allowing the detection of the presence of admixture in a population C from two other populations, A and B. If the value F 3 (C; A, B) is negative, then C does not appear to form a simple tree with A and B, but instead appears to be a mixture of A and B. If F 3 is zero, it indicates the absence of admixture, while a positive F 3 value implies simple tree-like relations among A, B and C (however, it does not reject admixture). Because of the complex genetic ancestry of human populations, there usually exists more than one possible model for any studied case. The f 4 -statistics has been developed in order to test alternative hypothetical trees [17]. The f 4 -statistics is used to estimate the admixture proportion of a test population (PC) under the assumption that we have a correct historical model. In this test, PA and PB are the potential contributors and PI is a reference population with no direct contribution to PC.
Admixture analysis is based on the maximum likelihood estimation of individual ancestries from multi-locus SNP genotype datasets [9,18]. It estimates the best possible sources and proportions of admixing components for any hypothetical number (K) of admixing sources. Visualization of admixture components offers an insight into the genetic structure of the studied populations.
Because there is no publicly available WGS data from modern Hungarians and since our results indicate that the use of exome data is suitable for population genetic analysis, we carried out population genetic analysis of modern Hungarians based on their exome data (HUN EXOME dataset). Based on our results, we also evaluated a strategy to filter exome data and offer the most suitable parameters for using them in population genetic analysis.

Results
The BEADCHIP dataset contained markedly higher amount of linked markers Some population genetic approaches like PCA or admixture analysis are based on the assumption of linkage equilibrium. Thus, it is important that linked markers are pruned from the datasets as linkage disequilibrium (LD) between tightly linked markers causes certain haplotypes to be more frequent than expected and large blocks of markers in complete LD can seriously distort the eigenvector/eigenvalue structure [7]. This is especially important in the exome dataset as exons of genes, or genes could be tightly packed in small, transcriptionally active chromosome regions. Therefore, we altered the recommended 50 kb sliding window [9] in the -indep-pairwise algorithm of Plink to 10,000 kb, while maintaining the recommended 10 SNPs increment and r 2 threshold of 0.1. The variant counts and the effect of LD pruning on the different datasets are summarized in Additional file 1: Table S1.
F ST values based on the BEADCHIP dataset are systematically overestimated between populations with larger genetic distance For each dataset, we calculated the pairwise F ST value between each studied population and compared the results of the different datasets (Additional file 2: Table  S3). In general, the F ST distances generated from the GENOME and EXOME datasets were found to be nearly identical. However in the EXOME dataset we found very small but systematic differences between the F ST values of African (except in the LWK African population) and European populations and the African and East Asian populations. We observed that F ST values calculated on the basis of the BEADCHIP dataset were systematically overestimated between populations originating from different super-populations.
Eigenvalues are notably larger for the BEADCHIP dataset compared to GENOME and EXOME datasets in PCA For each dataset, we performed the PCA analysis of all samples without outlier removal. The different datasets show a remarkably similar overall picture by the first two eigenvectors (Fig. 1a-c). The relative positions of the super-populations are almost the same, and we can even pinpoint several outlier individuals for example, some individuals with African ancestry, marked by red dots in the middlethat are positioned in a very similar pattern in each dataset. The greatest difference is that the absolute values of eigenvectors are significantly larger for the BEADCHIP dataset compared to the GENOME and EXOME datasets, while the EXOME dataset has the most similar eigenvector values to the GENOME dataset. The complete logs of all PCA analyses on the different datasets (containing detailed Tracy-Widom statistics, average divergence between populations, ANOVA statistics for population differences along each eigenvector, statistical significance of differences between populations and list of eigen best SNPs) are summarized in Additional file 1: DATA/PCA. According to the detailed statistics included in the logs the three datasets showed similar statistical power to differentiate populations.
In order to investigate whether the BEADCHIP and EXOME datasets represent similar population relations within each super-population in comparison to the GENOME dataset, next we performed the PCA analysis restricted to each super-population using default outlier removal (removing individuals with > 6 SD of topk eigenvectors). The detailed PCA of the populations for each super-population showed that the indicated eigenvectors and values were very similar in all three datasets ( Fig. 2a-e). The greatest difference between the three datasets was seen in the AFR super-population. The differences between the overlap of the historically known admixed ASW and ACB African populations and their relations to the other African populations indicated slightly different population affiliations in the BEAD-CHIP dataset compared to the GENOME and EXOME datasets.

f 3 -statistics of BEADCHIP dataset deviates from GENOME dataset
In order to compare the usability of the three datasets we calculated the f 3 -statistics for all possible combinations of population triads and plotted the resulting F 3 values Fig. 3. Figure 3a shows that the EXOME F 3 values is almost identical to GENOME results (Pearson correlation r = 0.9998), while the BEADCHIP data ( Fig. 3b) presents less correlation (r = 0.9911) with the F 3 values calculated from the GENOME dataset. The differences are confined to the larger positive F 3 values in our analyzed populations (shown as deviating red dots from green dots in Fig. 3b). The most deviating cases were those where F 3 value were calculated between any two East Asian populations in relation to an arbitrary African population.

F 4 (TSI, X; CHB; YRI) values were comparable in all analyzed datasets
In the analyzed populations, all possible combinations of any four populations result in an exceedingly large in number. In most cases, the relations between these population combinations would be meaningless. Therefore, in order to test potential bias between the different datasets, we only calculated the f 4 -statistic corresponding to a well-known East Eurasian-like ancestry of Northern European populations [19]. The F 4 (TSI, X; CHB; YRI) values where X denotes all possible test populations were calculated for each dataset (Additional file 1: Figure S1 A-C).
In all analyzed populations, the f 4 -statistics showed nearly identical East Asian and African components for each dataset. A population with negative values indicates East Asian gene flow, while positive values indicate dominant African genetic components in the test population. The order of populations was the same for all datasets and the relative ratios between the F 4 values of different populations was nearly identical. The absolute values were significantly higher in the BEADCHIP dataset compared to the GENOME dataset, while the EXOME dataset was more similar to it.

Admixture analysis reveals subtle differences between the different datasets
We performed the admixture analysis and calculated the Cross Validation (CV) error for different models (K = 3 to 10) for each dataset. Since the absolute CV error values were significantly higher in the BEADCHIP dataset compared to the GENOME and EXOME datasets, we displayed the relative CV values compared to the lowest fitting model (K = 3) that resulted the highest CV error for each dataset (Additional file 1: Figure S2). The best fitting model of admixture indicated by the minimum of cross validation error was K = 6 in the GENOME, EXOME and HUN-EXOME datasets, however the CV error of the BEADCHIP dataset indicated the K = 9 model as the best fitting model of admixture. The curve of the CV errors of the BEADCHIP dataset shows that this dataset resulted in very similar alternative models (ranging from K = 7 to 10) with almost identical CV errors. Since the analysis of the different dataset suggested different best fitting admixture models, we visualized both models (K = 6 and K = 9) for each dataset.
The analysis of the best fitting admixture model (K = 6) suggested by both the GENOME and EXOME datasets produced very similar admixture results (Additional file 1: Figure S3). Each color represents a different admixture component (K). The major topology and admixture components were very similar in each dataset for all analyzed populations however, the BEADCHIP systematically overestimated the minor admix components compared to the other two datasets (for example, South Asian component /marked as orange/ in Italian (TSI) or British (GBR) populations denoted by arrows in Additional file 1: Figure S3). The analysis of the best fitting admixture model (K = 9) suggested by the BEADCHIP dataset produced very similar admixture results for each datasets (Additional file 1: Figure S4). The major topology and admixture components were very similar for each dataset. The major differences compared to the K = 6 model were that in each dataset, the European super-population was split into two and the African super-population was split into three admixing components. The EXOME dataset was again most similar to the GENOME dataset, and again, the admixture of the BEADCHIP dataset systematically overestimated the minor admix components compared to the other two datasets (some examples are highlighted by arrows in Additional file 1: Figure S4).

Overrepresentation of AIMs in the BEADCHIP dataset
Our previous population genetic analyses suggested that the variant composition of the BEADCHIP dataset is different from the GENOME and EXOME datasets. To test this hypothesis we calculated the variance of minor allele frequencies (MAF) of the analyzed populations for each SNP and visualized it as a density plot (Fig. 4). Figure 4 shows that SNPs that are highly variable between the test populations are overrepresented in the BEADCHIP dataset, while the distribution observed in the EXOME dataset is nearly identical to the distribution observed in the GENOME dataset. Thus, our analysis also confirms that EXOME dataset does not suffer from the same bias as the BEADCHIP dataset given correct processing of raw sequencing data for which we provide a step-by-step recommendation in Fig. 5.

Analysis of the HUN-EXOME dataset
The PCA of the HUN-EXOME dataset (Additional file 1: Figure S5A) shows that Hungarians (denoted by purple circles) belong to the European super-population and that Hungarians are not in close relationship with the other European populations available in the public HGP dataset (Additional file 1: Figure S5B).
In the case of Hungarians the F 4 (TSI,HUN;CHB;YRI) value (Additional file 1: Figure S6) showed that Hungarians have higher East Asian genetic components than the Fig. 3 Comparison of F3 values obtained from the GENOME, EXOME and BEADCHIP datasets. F3 values were ordered and plotted relative to all possible combinations of population triads. a) F3 values from the EXOME vs. the GENOME dataset b) F3 values from the BEADCHIP vs. the GENOME dataset. The arrow denotes substantial deviations from the GENOME F3 values British population (GBR), but these genetic components are significantly smaller than those in the Finnish population (FIN).
Since the analysis of the EXOME dataset was comparable to the other datasets, we also performed the admixture analysis of the HUN-EXOME dataset for both the K = 6 and K = 9 models.
In the K = 6 model Hungarians were again classified into the European super-population as the major admixture component (depicted as blue) is the same as observed in other European populations (Additional file 1: Figure S7A). Within the Hungarian population we can observe a few individuals with significant South Asian genetic components (denoted by orange). In the dataset of analyzed populations considering the K = 9 admixture model (Additional file 1: Figure S7B), Europeans display a North-South gradient by the indicated two European specific admix components (represented by the Finnish and Italian populations). According to their geolocation Hungarians are situated in the middle of this gradient having approximately 50-50% portion of these admix components.

Discussion
The WGS, the WES, and the array-based datasets are the three main types of human genetic datasets available today. In this study, we compared the use of these SNP-selection strategies for population genetic analysis, as each method differs in terms of the ratio in which it contains variants under natural selection. Our GEN-OME dataset mainly contains non-exonic variants, since more than 98% of the human genome consists of non-exonic region and our coordinate-based selection was random. The EXOME dataset contains both non-exonic (~50%) and exonic variants (~50%), although only a portion of the exonic variants are functional. In order to make the various approaches comparable, we used the same curated HGP 1 kG genomic variant data to select the subsets of the GENOME, EXOME and BEADCHIP datasets (see in detail in the Methods section). The number of variants was comparable in all unpruned datasets (Additional file 1: Table S1).
Proper LD pruning is a crucial step prior to PCA analysis, as large blocks of completely linked markers may  introduce bias that could result additional eigenvectors [7]. Furthermore, it is also important in admixture analysis, because the calculations assume linkage equilibrium among the markers [9]. We observed that the unpruned BEADCHIP dataset contained slightly more variants than the GENOME dataset (567 k vs. 483 k variants), but most of them were tightly linked, as onlỹ 72 k markers (~12%) remained after LD pruning, while in the GENOME dataset the ratio was~43% (~205 k markers) which indicates a smaller fraction of linkage. We suppose that these differences are contributed to the tightly linked pre-selected AIMs in the BEADCHIP dataset. The coordinate-based EXOME dataset had somewhat higher linkage than the dispersed GENOME dataset. This is assumed to be a consequence of the organization of the human genome, where genes and exons are not homogeneously dispersed, but rather tend to be packed tightly in functionally active euchromatic chromosome regions. Correspondingly, after LD pruning about 149 k, a slightly lower proportion of variants (~38%) remained in the EX-OME dataset out of the~405 k unpruned variants. Comparing the PCA results of the EXOME dataset with the gold standard GENOME dataset, we refined the LD pruning parameters of exome data. We suggest extending the pruning window (to 10,000 kb)while keeping the original 0.1 squared correlation thresholdin order to counter the effect of the packed exome variant composition and to eliminate the tightly linked markers. According to our results, this modification did not significantly alter the variants of the BEADCHIP dataset; however, it did eliminate additional tightly linked variants in the EXOME and to a lesser degree in the GENOME datasets Additional file 1: Table S1.
The fixation index is one of the most commonly used statistics in population genetics, which is a measure of population differentiation due to genetic structure. The F ST distances between African (except the LWK population) and the European populations were very slightly (0.001-0.002) but systematically smaller using the EXOME dataset. On the other hand, the F ST values between the African and East Asian populations were very slightly (0.001-0.002) but systematically larger compared to the GENOME F ST distances. Since this slight difference was systematic between the random GENOME and the EXOME dataset (which by definition contains functional variants besides the non-coding and other functionally inert variants), we hypothesize that a portion of functional variants are accountable for this phenomenon. However, the deviation (~1-3%) is still only a portion of what was observed in the BEADCHIP dataset. The comparison of F ST values of the BEADCHIP dataset to the GENOME dataset revealed that the pairwise F ST distances between populations of different super-populations were systematically larger (~1-12%), and that the extent of the difference appears to be correlated to the phylogenetic distance.
On the contrary, we detected almost no differences between the F ST distances of populations within the same super-population, except in the highly admixed AMR super-population. We assume that this is due to a general overrepresentation of differentiating SNPs (AIMS) and imbalances in the selection and proportion of the marker composition in the pre-selected BEADCHIP dataset. This hypothesis is also supported by the observed F ST values of the admixed ASW population. The ASW population is a sub-population of African Americans in the Southwestern United States who originated from West-Africa, and later mixed with Caucasian and American Indians. Accordingly, the BEADCHIP data places this population closer to its admix sources -the European (EUR) and Admixed American (AMR) super-populations -than those of the GENOME F ST values, indicating that the overrepresentation of AIMs distorts the true population distances. As F ST can be used to estimate coalescence times [20,21], significant deviations in F ST values -such as observed using the BEADCHIP datasetmay lead to bias in these estimations.
For each dataset the PCA analysis showed both similar topology and comparable relations between the analyzed populations. We observed that for the first two eigenvectors of the whole dataset (Fig. 1a-c) the eigenvalues were greater in the BEADCHIP dataset compared to the GENOME dataset, while the EXOME datasets were more similar to it. The eigenvalue with the largest absolute value is known as the dominant eigenvalue and can be used to determine the rate of growth in the population [22]. An overestimation of the eigenvalues miscalculates the population growth rate. The largest differences between the detailed PCA were seen in the African super-population (Fig. 2e) where the BEADCHIP dataset gave slightly different eigenvalues and population relations compared to the GENOME and EXOME datasets. Similarly to the F ST results, the admixed ASW and ACB populations displayed slightly different relations to other populations. We assume that again, the overrepresentation of AIMs in the BEADCHIP dataset is responsible for the increased eigenvalues and the different relations of the admixed ASW and ACB populations. Nonetheless, the genetic relationship among the studied populations was still comparable.
Comparison of the three datasets showed that the GENOME and the EXOME data gave almost identical F 3 values (r = 0.9998 Fig. 3a), while the Z scores were slightly smaller in the EXOME dataset, which was attributable to the smaller SNP count. On the other hand, the f 3 -statistics of the BEADCHIP dataset showed less correlation (r = 0.9911 Fig. 3b). The Z scores of the f 3 -statistics in the BEADCHIP dataset were also higher, even though the SNP count was about one third of the GEN-OME dataset, which indicates higher deviation from the mean. Plotting the corresponding F 3 values revealed systematic differences in a large portion of population combinations between the BEADCHIP and GENOME datasets. Investigation of F 3 values showed that tree like split of East Asian populations from African populations was systematically overestimated by the f 3 -statistics based on the BEADCHIP dataset. We assume that this bias is due to the overrepresentation of AIMs between the East Asian and African populations within the pre-selected variants of the BEADCHIP dataset. Taking all this together, we conclude that the EXOME data is highly suitable for f 3 -statistics. Although the absolute F 3 value deviation from zero is meaningless if we only test whether f-statistics is consistent with zero [8] (meaning no admixture), the inferred magnitude of relatedness in comparison to relations between other populations may lead to bias or overinterpretation of data in some specific cases (such as where markers are not evenly represented in the analyzed populations) of the BEADCHIP dataset (due to higher F 3 values and optimistic Z scores).On the other hand our analysis supports that f 4 -statistics is robust to SNP ascertainment (all three datasets resulted in nearly identical proportions) and deviation from zero is only observed if the test population is admixed [8].
The admixture analysis of different datasets suggest different best-fit admix modelsSince the BEADCHIP dataset resulted in higher CV errors and a number of very similar alternative models, it appears that this dataset is less conclusive for pinpointing the true admixture model. Nevertheless, the admixture analysis of the different datasets resulted very similar admixture components for all of the tested models (Additional file 1: Figures S3-S4). The observed overrepresentation of minor admixture components in the BEADCHIP dataset may lead to overestimation of their admix ratios.
In our comparative analyses, we used the same HGP dataset to make an unbiased assessment of the different strategies of variant selection. However, comparing datasets from different sources may lead to bias due to the differences in the applied NGS variant calling tools, pipelines, thresholds, and quality of sequences. The majority of genotyping uncertainties stem primarily from the fact that various NGS pipelines use slightly different (only partially overlapping) quality parameters and pipeline specific fix tresholds which is not always optimal for analyzing all genomic regions with different read depths (due to inter-sample and/or target region variances).. Thus, in order to minimize the bias, we applied a number of criteria for exome data processing before using them for population genetic analyses. Hence, we excluded the low-coverage data, based on the real coverage profile of our aligned NGS reads, as these data may have led to ambiguous variant calls. We excluded all read length variations (INS/DELS/DUPS) from the analysis since the same variant may be represented ambiguously by different variant callers. We also excluded potentially pseudogenic, conservative and repetitive regions where reads could be ambigiously mapped to multiple genomic regions and the proportion of the alternatively aligned sequencing reads may lead to differences depending on the threshold values or the pipeline applied for the analysis.
Using these strict criteria, we merged the Hungarian exome data and analyzed the resulting HUN-EXOME dataset with the appropriate population genetic tools. Both the PCA and admixture analysis of the modern Hungarian exome dataset confirms that genetically, modern Hungarians are Europeans (Additional file 1: Figure S5 PCA; Additional file 1: Figure S7 K = 6, K = 9 admixture plots). We note here that our admixture analysis (K = 9) indicated two genetic components with a North-South European gradient which has a~50-50% portion in Hungarians. Admixture analysis also detected a portion of a South Asian component in a few individuals of the Hungarian population, which in our view can be attributed to the Gypsies living as an ethnic minority (~5%) in Hungary. F 3 analysis of HUN-EXOME dataset also suggests a small but significant (high Z scores) South-Asian-HUN admixture (Additional file 1: Table S2).
Historically, gypsy tribes left India in the 9th and 10th centuries as a result of Muslim attacks in areas they inhabited and first appeared in territory of the medieval Kingdom of Hungary in the 14th and 15th centuries, probably fleeing from the conquering Turks in the Balkans [23]. This assumption is also supported by the fact that the South-Asian genetic component is confined to only a few Hungarian individuals with high contributions indicating a recent admixture. Unfortunately we cannot explicitly verify the ethnic origin as our exome data was anonymous and therefore we had no information on ethnicity. Modern Hungarians identify themselves as having originated from the Hungarian Conquerors, who are deemed to have arrived to the Carpathian Basin in the ninth Century migrating from around the Ural Mountains, which is the natural border between Europe and Asia. The f 4 -statistic indicated a small East Asian admixture component in modern Hungarians (Additional file 1: Figure S6) that was also seen as minor admixture components in few Hungarian individuals (Additional file 1: Figure S7). The minor East Asian component detected in modern Hungarians is possibly the genetic trace of Hungarian Conquerors as also suggested by mtDNA analysis [24,25]. Because the publicly available HGP dataset contains worldwide populations with no or very little genetic relation to modern Hungarians, our analysis indicated only known and plausible relations of modern Hungarians in relation to the analyzed populations (such as general European ancestry with small-scale East-Asian components and a recent admixture with South-Asian components). However, this systematic analysis confirms the usability of WES data in population genetic analysis and leads us to conclude that exome data from populations having shared ethnogenesis with Hungarians could result in an even better understanding of their genetic history.

Conclusion
Overall, our comparative analysis indicates that the array-based preselected SNP-set (BEADCHIP dataset) deviates most from the GENOME dataset. The reason behind this phenomenon may be twofold. First, the pre-selection of SNPs in the array is not necessarily a uniform representation of all of the analyzed populations, and second, the increased proportion of AIMs is shifting the balance towards highlighting the differences between populations. The increased proportion of AIMs makes the BEADCHIP dataset suitable for sensitive exploration of admixture components. However, it could also lead to deviations of the Fixation index, specific cases of f3-statistics, PCA eigenvalues, suggested admixture model, and admixture rates. Thus, all derived parameters (e.g. coalescent time, population growth rate) estimated from these statistics are prone to bias in case of array-based data. WES data, on the other hand, is not affected by this bias and is suitable for population genetic analyses. Based on the usability of the EXOME dataset we would encourage everyone to use and to publicly share WES sequences with the correct indication of ethnic and geographical origin, which could contribute towards a better understanding of the genetic relationships among human populations.

Preparation of Hungarian exome data
Our previously published BAM files of Hungarian whole exomes [26] were aligned by the BWA-MEM algorithm [27]. Using the whole cohort, we identified the high coverage regions (all samples in the cohort had coverage >10x) using samtools bedcov (version 1.3.1) algorithm [28] with the SureSelect V5 all exon plus UTR kit manifest bed coordinates. Since some of the regions may contain repetitive elements or pseudogenic regions with non-unique sequences, we excluded all regions that had any MAPQ0 reads (mapping quality 0, indicating that the read could be mapped to multiple genomic regions). We excluded sex chromosomes from the analyzed regions. As a result, we generated a BED coordinate list (Additional file 1: Data/HighCov_HighQual_EXOME.bed) that contained the high coverage, unique genomic regions of the exome kit. Variant calling was performed by the GATK HaplotypeCaller (version 3.5) best practice [29] using the parameters -stand_emit_conf 10 -stand_call_conf 30 in the HaplotypeCaller module, and --ts_filter_level 99.0 in the variant recalibration (ApplyRecalibration) module. We filtered the variants to include only high quality SNPs (PASS filter) in the final high coverage/unique bed regions. We also filtered out multi-allelic variants and variants that had < − 0.5 InbreedingCoeff (indicating variants that violate HW equilibrium, which would potentially be technical errors since our cohort consisted of unrelated patients).

Preparation of public datasets
We used the publicly available variants from the higly currated VCF files of the Human Genome Project 1 kG phase 3 dataset [30]. We excluded the geographically inaccurate To compare the capabilities of the sequencing-based (WES and WGS) and array-based approaches, we created three SNP datasets (denoted as EXOME, GENOME and BEADCHIP) established upon the following rules: first, the EXOME dataset (Additional file 1: Data/EX-OME) was prepared by filtering the public 1 kG variants with the bedtools intersect algorithm using the genomic coordinates of the high-coverage high-quality exome BED coordinate list that we established using the Hungarian exome data. Second, the GENOME dataset was created on the basis of a homogeneously dispersed genomic coordinate list that spanned all somatic chromosomes (Additional file 1: Data/GENOME.bed). The first and last 5 Mb of the telomeric regions of chromosomes were excluded in order to eliminate uncertain sequences, such as repetitive elements. The remaining genomic regions on each chromosome was equally divided; the first 258 base pairs in every 10 kb DNA stretch were used by the bedtools intersect algorithm for extracting homogeneously dispersed variants across the genome (Additional file 1: Data/GENOME). The resulting unbiased genomic region coordinates were found to have a cumulative size (68.7 Mb), which is similar to the cumulative size of the high-coverage exome regions (68.9 Mb).
In order to prepare the BEADCHIP dataset (Additional file 1: Data/BEADCHIP), the genome coordinates of a frequently used Human genotyping array kit (Illumina Human610-Quad BeadChip) were used from the kit's manifest file and converted to BED coordinates. The bedtools intersect algorithm was used to filter the corresponding variants from the 1 kG genomic variants. From all three datasets, we removed all multi allelic variants, along with variants that were found in less than 10 individuals (or in all but 10 individuals in case of refseq error). This resulted in comparable variant numbers for each dataset (567 k BEADCHIP, 405 k EXOME, 483 k GENOME). The 1 kG annotated joint VCF was re-coded to plink.ped and plink.map formats. Plink (version 1.9) was used to generate the binary bed format files used in the downstream analysis [31].

Merging Hungarian exome data with public dataset
In order to create the merged HUN-EXOME dataset, we used the same high-coverage high-quality exome BED coordinate list, which we established using Hungarian exome data, and the bedtools intersect algorithm for filtering the variants in both the Hungarian exome data and the 1 kG datasets. The filtered exome and 1 kG variants were merged with the GATK CombineVariants algorithm. We removed multi allelic variants, as well as variants that were only found in less than 10 individuals (or all but 10 individuals in case of refseq error). The resulting joint VCF were re-coded to plink.ped and plink.map formats and we used plink to generate the binary bed format file (Additional file 1: Data/HUN-EXOME).

LD pruning of datasets
LD pruning was carried out using the -indep-pairwise algorithm of plink (version 1.9). Because of the structure of the human genome, it was expected that exome variants would yield non-homogeneously dispersed markers. Therefore, we performed the LD pruning using different sliding window sizes (the recommended 50 kb of admixture protocol, and an extended 10,000 kb) to prune linked markers in order to identify the best parameters. For both window sizes, we kept the recommended 10 SNPs increment and r 2 threshold of 0.1 to allow pruning of markers by the same linkage criteria. In order to allow unbiased comparison of the three methods, we used the same LD pruning parameters (10,000 kb sliding window, 0 SNPs increment and r 2 threshold of 0.1) for each dataset.

F ST and PCA calculations
The pairwise F ST matrix and the PCA analysis of populations were performed by the EIGENSOFT software (version 6.1.3) [7] on the LD pruned datasets. For each subset of data (whole dataset and the different super-populations), the LD pruning of linked variants and the subsequent PCA calculations were performed separately. PCA analysis was carried out without outlier removal for the whole datasets and with outlier removal (SmartPCA config file, outliersigma tresh SD = 6) for the analysis of individual populations for each super-population. PCA plots were visualized by ggplot2 (version 2.2.1) [32].

and f 4 -statistics
The F 3 tests were carried out by the qp3Pop program of ADMIXTOOLS [8] for each population triad of the analyzed populations. The F 4 tests were calculated using the fourpop algorithm of TreeMix software (version 1.13). For each dataset, the corresponding F 4 (TSI,X;CHB,YRI) values were calculated (where X refers to the given test population). F 3 and F 4 values of the tests were visualized by ggplot2.

Admixture analysis
Admixture analysis was performed by the ADMIXTURE software (version 1.3.0) using the LD pruned datasets with the --cv option for K = 3 to 11 values using 20 iterations and randomized seeds [9]. The best admixtures suggested by the cross-validation plots of genome/exome and BEADCHIP datasets were visualized by a custom Perl script using Linux ImageMagick software (version 6.7.7-10).

Additional files
Additional file 1: Table S1. Number of high-quality biallelic variants in GENOME, EXOME, BEADCHIP, and HUN-EXOM datasets before and after LD pruning. Table S2. f3-statistics of Hungarian and selected South Asian populations indicate admixture which could be attributed to the Gypsies living as an ethnic minority in Hungary. Figure S1. The f4 (TSI, X; CHB; YRI) statistics estimates the East Asian and African components for each dataset. Negative values indicates East Asian gene flow, while positive values indicate dominant African genetic components. Figure S2. Calculated CV error for different admixture models (K=3 to 10). The best fitting model of admixture indicated was K=6 in the GENOME, EXOME and HUN-EXOME datasets, however, it was K=9 in case of the BEADCHIP dataset. Figure S3. Admixture analysis of 25 populations with the K=6 admixture model. The major topology and admixture components were very similar in each dataset for all analyzed populations but the minor admix components were systematically overestimated using the BEADCHIP dataset. Figure S4. Admixture analysis of 25 populations with the K=9 admixture model. Besides the minor admix components overestimation using BEADCHIP this admixture model suggested that the European super-population was split into two and the African super-population was split into three admixing components. Figure S5. PCA of the HUN-EXOME dataset confirms that genetically, modern Hungarians belong to the European super-population and within the Europeans, two genetic components exist with a North-South European gradient. Figure S6.